Shi Feng, Liu Ying, Li Min, Wen Peng, Qian Qiu Qin, Fan Yibin, Huang Ruixue
Internal Medicine Department of Thyroid and Radionuclide Therapy, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, People's Republic of China.
Zhejiang provincial people's hospital, People's Hospital of Hangzhou Medical College, Hangzhou, People's Republic of China.
Dose Response. 2019 Jul 25;17(3):1559325819864223. doi: 10.1177/1559325819864223. eCollection 2019 Jul-Sep.
Occupational exposure of radiation among medical radiation workers contributes to the subsequent increased risk of thyroid cancer. Long noncoding RNAs (lncRNAs) are emerging as important regulators of cancer biology. However, little is known about lncRNA expression in thyroid cancer tissues from patients who are exposed to medical occupational radiation. The purpose of this study is to reveal the transcriptomes difference between thyroid cancer tissues and adjacent nonneoplastic thyroid tissues.
Microarray technology was used in this study. Quantitative reverse transcription polymerase chain reaction was adopted to verify 6 differentially expressed lncRNAs. Gene ontology and pathway analyses were performed using standard enrichment computational methods. Potential target genes of the differentially expressed lncRNAs were predicted with 2 independent algorithms.
A total of 23 lncRNA and messenger RNA transcripts were found differentially expressed in the thyroid cancer tissues (fold change ≥2.0, < .05). This differential lncRNA expression may affect many pathways, including those involved in cysteine and methionine metabolism, Huntington disease, propanoate metabolism, and carcinogenesis.
Our study provides a transcriptome-wide screening and analysis of the lncRNA expression profile in thyroid cancer tissues from patients with medical occupational radiation exposure and lays the foundation for further investigation of lncRNAs related to thyroid cancer development and carcinogenic risk of medical occupational radiation exposure.
医学放射工作人员的职业性辐射暴露会导致后续甲状腺癌风险增加。长链非编码RNA（lncRNA）正成为癌症生物学的重要调节因子。然而，对于职业性接触医学辐射患者的甲状腺癌组织中lncRNA的表达情况知之甚少。本研究的目的是揭示甲状腺癌组织与相邻非肿瘤性甲状腺组织之间的转录组差异。
本研究采用微阵列技术。采用定量逆转录聚合酶链反应来验证6种差异表达的lncRNA。使用标准富集计算方法进行基因本体论和通路分析。用2种独立算法预测差异表达lncRNA的潜在靶基因。
共发现23种lncRNA和信使RNA转录本在甲状腺癌组织中差异表达（倍数变化≥2.0，<0.05）。这种lncRNA差异表达可能影响许多通路，包括那些参与半胱氨酸和蛋氨酸代谢、亨廷顿病、丙酸代谢和致癌作用的通路。
我们的研究提供了职业性接触医学辐射患者甲状腺癌组织中lncRNA表达谱的全转录组筛查和分析，为进一步研究与甲状腺癌发生及职业性接触医学辐射致癌风险相关的lncRNA奠定了基础。
