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使用实时多重定量聚合酶链反应法同时检测沙眼衣原体和淋病奈瑟菌

Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay.

作者信息

Eboigbodin Kevin E

机构信息

Research and Development, Orion Diagnostica Oy, Espoo, Finland.

出版信息

Methods Mol Biol. 2019;2042:27-32. doi: 10.1007/978-1-4939-9694-0_4.

DOI:10.1007/978-1-4939-9694-0_4
PMID:31385268
Abstract

Real-time polymerase chain reaction (qPCR) has become a prominent technique in life science research particularly for the detection and monitoring of biomarkers, pathogens, and environmental contaminants. Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are among the major pathogens responsible for sexually transmitted diseases (STDs). Here, multiplex qPCR was utilized for the amplification and detection Chlamydia trachomatis and Neisseria gonorrhoeae within the same reaction tube.

摘要

实时聚合酶链反应（qPCR）已成为生命科学研究中的一项重要技术，尤其用于生物标志物、病原体和环境污染物的检测与监测。沙眼衣原体（CT）和淋病奈瑟菌（NG）是导致性传播疾病（STD）的主要病原体。在此，多重qPCR被用于在同一反应管中扩增和检测沙眼衣原体和淋病奈瑟菌。

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