Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay.

Real-time polymerase chain reaction (qPCR) has become a prominent technique in life science research particularly for the detection and monitoring of biomarkers, pathogens, and environmental contaminants. Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are among the major pathogens responsible for sexually transmitted diseases (STDs). Here, multiplex qPCR was utilized for the amplification and detection Chlamydia trachomatis and Neisseria gonorrhoeae within the same reaction tube.