Abbott m2000 RealTime 与 Alinity m STI 系统检测沙眼衣原体、淋病奈瑟菌和生殖支原体的比较。
Comparison between Abbott m2000 RealTime and Alinity m STI systems for detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycoplasma genitalium.
机构信息
Department of Clinical Microbiology, Uppsala University Hospital, SE-751 85, Uppsala, Sweden.
Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
出版信息
Eur J Clin Microbiol Infect Dis. 2021 Oct;40(10):2217-2220. doi: 10.1007/s10096-020-04135-9. Epub 2021 Mar 15.
Abstract
The new Abbott Alinity m STI Assay was compared with Abbott m2000 RealTime PCR. For Chlamydia trachomatis, 26 (7.5%) of 347 samples were positive in the Alinity assay and 24 (6.9%) in the m2000 assay. Corresponding figures for Neisseria gonorrhoeae were 23 (6.6%) and 17 (4.9%). For Mycoplasma genitalium, 22 (7.9%) of 279 samples were positive in the Alinity assay and 18 (6.5%) in the m2000 assay, for which DNA extraction was performed on an m2000sp instrument combined with in-house real-time PCR. The Alinity assay has at least the same sensitivity as the m2000 assay. The specificity was evaluated by discrepancy analysis.
摘要
新的雅培 Alinity m STI 检测法与雅培 m2000 实时 PCR 法进行了比较。对于沙眼衣原体，在 Alinity 检测法中，347 个样本中有 26 个（7.5%）为阳性，在 m2000 检测法中，24 个（6.9%）为阳性。淋病奈瑟菌的相应数字分别为 23 个（6.6%）和 17 个（4.9%）。对于解脲支原体，在 Alinity 检测法中，279 个样本中有 22 个（7.9%）为阳性，在 m2000 检测法中，18 个（6.5%）为阳性，对于 m2000sp 仪器上提取的 DNA，采用了内部实时 PCR 法。Alinity 检测法的灵敏度至少与 m2000 检测法相同。通过差异分析评估了特异性。
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本文引用的文献
1
Environmental Contamination by Chlamydia trachomatis RNA Can Cause False-Positive Test Results in Clinical Samples.环境中沙眼衣原体 RNA 的污染可能导致临床样本的假阳性检测结果。
Sex Transm Dis. 2021 Jul 1;48(7):e88-e90. doi: 10.1097/OLQ.0000000000001323.
2
Prevalence of by anatomical site in men who have sex with men: a systematic review and meta-analysis.男男性行为者中按解剖部位分布的患病率：系统评价和荟萃分析。
Sex Transm Infect. 2020 Dec;96(8):563-570. doi: 10.1136/sextrans-2019-054310. Epub 2020 Apr 27.
3
Gonorrhoea.淋病。
Nat Rev Dis Primers. 2019 Nov 21;5(1):79. doi: 10.1038/s41572-019-0128-6.
4
Confirmatory assays are essential when using molecular testing for Neisseria gonorrhoeae in low-prevalence settings: insights from the third National Survey of Sexual Attitudes and Lifestyles (Natsal-3).在低流行率环境中使用淋病奈瑟菌分子检测时，确证检测至关重要：来自第三次全国性态度与生活方式调查（Natsal-3）的见解
Sex Transm Infect. 2015 Aug;91(5):338-41. doi: 10.1136/sextrans-2014-051850. Epub 2014 Dec 15.
5
Trichomonas vaginalis infections are rare among young patients attending an STI clinic in Sweden.在瑞典一家性传播感染诊所就诊的年轻患者中，阴道毛滴虫感染较为罕见。
Acta Derm Venereol. 2015 Mar;95(3):343-4. doi: 10.2340/00015555-1946.
6
2012 European guideline on the diagnosis and treatment of gonorrhoea in adults.2012年成人淋病诊断与治疗欧洲指南。
Int J STD AIDS. 2013 Feb;24(2):85-92. doi: 10.1177/0956462412472837.
7
Chlamydia and gonorrhoea contamination of clinic surfaces.诊所表面的衣原体和淋病污染。
Sex Transm Infect. 2012 Oct;88(6):418-21. doi: 10.1136/sextrans-2012-050543. Epub 2012 Apr 25.
8
Evaluation of screening tests for detecting Chlamydia trachomatis: bias associated with the patient-infected-status algorithm.沙眼衣原体筛查试验的评估：与患者感染状态算法相关的偏倚。
Epidemiology. 2012 Jan;23(1):72-82. doi: 10.1097/EDE.0b013e31823b506b.
9
'The difference in determinants of Chlamydia trachomatis and Mycoplasma genitalium in a sample of young Australian women'.《在澳大利亚年轻女性样本中沙眼衣原体和生殖支原体决定因素的差异》
BMC Infect Dis. 2011 Feb 1;11:35. doi: 10.1186/1471-2334-11-35.
10
A duplex Neisseria gonorrhoeae real-time polymerase chain reaction assay targeting the gonococcal porA pseudogene and multicopy opa genes.一种针对淋病奈瑟菌porA假基因和多拷贝opa基因的双重淋病奈瑟菌实时聚合酶链反应检测方法。
Diagn Microbiol Infect Dis. 2008 May;61(1):6-12. doi: 10.1016/j.diagmicrobio.2007.12.007. Epub 2008 Jan 14.
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