State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, China.
College of Food Science and Technology, Hainan University, Haikou, China.
J Sci Food Agric. 2019 Dec;99(15):6822-6832. doi: 10.1002/jsfa.9967. Epub 2019 Sep 18.
Angiotensin-converting enzyme (ACE) inhibitory peptides were found to alleviate acute hepatitis significantly. In this study, we purified and identified ACE inhibitory peptide from cashew to evaluate its protective role on alcohol-induced acute hepatitis in mice.
The ACE inhibitory peptides were purified by using consecutive chromatographic techniques. One of these peptides (FETISFK) exhibited the highest ACE inhibition rate (91.04 ± 0.31%). In vivo, the results showed that ACE inhibitory peptide decreased levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) caused by alcohol exposure. Moreover, it could increase the activities of superoxide dismutase (SOD) and glutathione (GSH), and decrease the level of malondialdehyde (MDA). It was also found to down-regulate markedly the expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). It could also decrease the expression of ACE, angiotensin II (AngII) and angiotensin II type 1 receptor (AT R).
These findings support the view that the ACE inhibitory peptide alleviated acute hepatitis by down-regulating the ACE-AngII-AT R axis, broadening the research approach to prevent acute hepatitis, and providing experimental data for the development and utilization of cashews. © 2019 Society of Chemical Industry.
血管紧张素转换酶（ACE）抑制肽被发现能显著缓解急性肝炎。在这项研究中，我们从腰果中分离和鉴定了 ACE 抑制肽，以评估其对小鼠酒精性急性肝炎的保护作用。
采用连续色谱技术对 ACE 抑制肽进行了纯化。其中一种肽（FETISFK）表现出最高的 ACE 抑制率（91.04±0.31%）。在体内，结果表明 ACE 抑制肽可降低酒精暴露引起的丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）水平。此外，它可以提高超氧化物歧化酶（SOD）和谷胱甘肽（GSH）的活性，降低丙二醛（MDA）水平。它还显著下调白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的表达。它还可以降低 ACE、血管紧张素 II（AngII）和血管紧张素 II 型 1 受体（AT R）的表达。
这些发现支持 ACE 抑制肽通过下调 ACE-AngII-AT R 轴缓解急性肝炎的观点，拓宽了预防急性肝炎的研究方法，并为腰果的开发利用提供了实验数据。© 2019 化学工业协会。
