Arise Rotimi Olusanya, Taofeek Oluwaseun Oluwatosin, Babaita Kehinde, Adeoye Raphael Idowu, Osemwegie Omorefosa
Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, P.M.B. 1515, Ilorin, Nigeria.
Department of Biological Sciences, College of Science and Engineering, Landmark University, Omu-Aran, Nigeria.
Heliyon. 2021 Mar 12;7(3):e06384. doi: 10.1016/j.heliyon.2021.e06384. eCollection 2021 Mar.
Several novel functional peptides have been successfully extracted from plant storage proteins. This study investigated the degree of hydrolysis, peptide yield, amino acid constituents, angiotensin converting enzyme (ACE), alpha amylase inhibitory and antioxidant activities of cashew () nut proteins (CNP) hydrolysates (CNPHs). Cashew nut proteins (albumin and globulin) were hydrolysed using pancreatin, Alcalase and trypsin. The peptide yield and degree of hydrolysis (DH) of CNP by pancreatin (75.69 ± 0.84%; 37.39 ± 0.31) was significantly higher than those by Alcalase (61.67 ± 0.55%; 23.87 ± 0.23) and trypsin (43.33 ± 0.45%; 11 ± 0.15). The inhibition of ACE by albumin and globulin hydrolysates was concentration dependent. At 1.2 mg/mL, ACE-inhibitory activity of pancreatic cashew nut globulin (CNGH) hydrolysate (51.65 ± 1.2%) was significantly higher than those of Alcalase (34.603 ± 0.65%) and tryptic (29.92 ± 0.73%) CNGHs. Cashew nut albumin hydrolysate (CNAH) demonstrated concentration-dependent alpha-amylase inhibition (IC 0.17 ± 0.02-0.41 ± 0.021 mg/mL). The order of inhibition was tryptic > Alcalase > pancreatic CNAHs. The pancreatic hydrolysates of both albumin and globulin fractions displayed the highest DPPH antioxidant activity, while pancreatic CNAH was the most potent superoxide anion scavenger. These findings therefore posit that cashew nut globulin and albumin hydrolysates are laden with useful bioactive peptides that may be further explored for regulation of blood pressure and sugar in hypertensive and diabetic models.
几种新型功能肽已成功从植物储存蛋白中提取出来。本研究调查了腰果（）坚果蛋白（CNP）水解产物（CNPHs）的水解程度、肽产率、氨基酸组成、血管紧张素转换酶（ACE）、α淀粉酶抑制和抗氧化活性。使用胰蛋白酶、碱性蛋白酶和胰蛋白酶对腰果蛋白（白蛋白和球蛋白）进行水解。胰蛋白酶对CNP的肽产率和水解程度（DH）（75.69±0.84%；37.39±0.31）显著高于碱性蛋白酶（61.67±0.55%；23.87±0.23）和胰蛋白酶（43.33±0.45%；11±0.15）。白蛋白和球蛋白水解产物对ACE的抑制作用呈浓度依赖性。在1.2mg/mL时，胰蛋白酶水解的腰果坚果球蛋白（CNGH）水解产物的ACE抑制活性（51.65±1.2%）显著高于碱性蛋白酶（34.603±0.65%）和胰蛋白酶水解的（29.92±0.73%）CNGHs。腰果坚果白蛋白水解产物（CNAH）表现出浓度依赖性的α淀粉酶抑制作用（IC 0.17±0.02 - 0.41±0.021mg/mL）。抑制顺序为胰蛋白酶>碱性蛋白酶>胰蛋白酶水解的CNAHs。白蛋白和球蛋白组分的胰蛋白酶水解产物均表现出最高的DPPH抗氧化活性，而胰蛋白酶水解的CNAH是最有效的超氧阴离子清除剂。因此这些发现表明，腰果坚果球蛋白和白蛋白水解产物富含有用的生物活性肽，可在高血压和糖尿病模型中进一步探索其对血压和血糖的调节作用。
