Collaborative Innovation Center of Zhejiang Green Pesticide, State key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, People's Republic of China.
Pest Manag Sci. 2020 Feb;76(2):747-757. doi: 10.1002/ps.5575. Epub 2019 Aug 31.
Emamectin benzoate (EB) has recently been successfully applied as a trunk injection for preventative control of the pine wilt disease (PWD) caused by Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle. Here, a whole-organism transcriptomic analysis provides comprehensive insights into the adverse effects of EB on B. xylophilus.
A large set of differentially expressed genes (DEGs) were found, demonstrating the antagonistic effects of EB on B. xylophilus embryonic and larval development, reproduction, nervous and motor systems, and pathogenesis. In toxicity assays with EB, the number of eggs laid, hatching rate, thrashing frequency, and developmental rate of B. xylophilus were significantly suppressed at low concentrations (0.1 μg mL ). Moreover, the transcriptional changes validated by real-time quantitative PCR showed downregulated transcript levels of the genes encoding pectate lyases, β-1,4-endoglucanases, and upregulated the genes encoding glutamate-gated chloride channel, γ-aminobutyric acid type β receptor, uridine 5'-diphospho-glucuronosyl transferase, ATP-binding cassette transporter. The potential responses of B. xylophilus to EB included the upregulation of several genes putatively contributing to oocyte protection, stem cell renewal, and xenobiotic degradation, implying the potential for drug resistance to develop.
Our findings further our understanding of the effects of EB for managing the PWD and may help to improve the pesticide-use strategies for controlling B. xylophilus. © 2019 Society of Chemical Industry.
埃玛菌素苯甲酸盐（EB）最近已成功应用于松材线虫（Bursaphelenchus xylophilus （Steiner & Buhrer）Nickle）引起的松材线虫病（PWD）的树干注射预防控制。在这里，全生物体转录组分析为 EB 对松材线虫的不良影响提供了全面的见解。
发现了大量差异表达基因（DEGs），表明 EB 对松材线虫胚胎和幼虫发育、繁殖、神经和运动系统以及发病机制具有拮抗作用。在 EB 的毒性试验中，低浓度（0.1μg/mL）显著抑制了松材线虫产卵数、孵化率、抽搐频率和发育率。此外，实时定量 PCR 验证的转录变化显示，编码果胶裂解酶、β-1,4-内切葡聚糖酶的基因转录水平下调，编码谷氨酸门控氯离子通道、γ-氨基丁酸型β受体、尿苷 5'-二磷酸葡萄糖醛酸基转移酶、ATP 结合盒转运蛋白的基因转录水平上调。松材线虫对 EB 的潜在反应包括上调几个可能有助于卵母细胞保护、干细胞更新和外来物降解的基因，这意味着可能会产生抗药性。
我们的研究结果进一步了解了 EB 管理 PWD 的效果，并可能有助于改善控制松材线虫的农药使用策略。© 2019 化学工业协会。
