Takai Kazuya, Suzuki Toshio, Kawazu Kazuyoshi
Development Department, Izutsuya Chemical Industry Co Ltd, 1-11-30 Hanazono, Kumamoto 860-0072, Japan.
Pest Manag Sci. 2004 Jan;60(1):42-8. doi: 10.1002/ps.777.
In an earlier paper the authors reported the creation of a novel emamectin benzoate 40 g litre(-1) liquid formulation (Shot Wan Liquid Formulation). The injection of this formulation exerted a preventative effect against the pine wilt disease caused by the pine wood nematode, Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle, and this effect lasted for at least 3 years. The present study was carried out to show experimentally that the marked effect of this formulation was due to the presence and persistence in pine tissues of sufficient amounts of emamectin benzoate to inhibit nematode propagation. A cleanup procedure prior to quantitative analysis of emamectin benzoate by fluorescence HPLC was devised. The presence of the compound in concentrations sufficient to inhibit nematode propagation in the shoots of current growth and its persistence for 3 years explained the marked preventative effect. Non-distribution of emamectin benzoate in some parts of the lower trunk suggested that the formulation should be injected at several points for large trees in order to distribute the compound uniformly to lower branches.
在一篇较早的论文中，作者报道了一种新型的40克/升甲氨基阿维菌素苯甲酸盐液体制剂（Shot Wan液体制剂）的创制。注射该制剂对由松材线虫（Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle）引起的松树萎蔫病具有预防作用，且这种作用持续了至少3年。开展本研究是为了通过实验表明该制剂的显著效果是由于在松树组织中存在并持续有足够量的甲氨基阿维菌素苯甲酸盐来抑制线虫繁殖。设计了一种在通过荧光高效液相色谱法定量分析甲氨基阿维菌素苯甲酸盐之前的净化程序。该化合物以足以抑制当年生嫩枝中线虫繁殖的浓度存在及其持续3年的情况解释了其显著的预防效果。甲氨基阿维菌素苯甲酸盐在树干下部某些部位未分布，这表明对于大树应在几个点进行注射，以便将该化合物均匀地分布到下部枝条。
