Sequani Limited, Bromyard Road, Ledbury, HR8 1LH, Herefordshire, United Kingdom.
Reprod Toxicol. 2019 Sep;88:91-128. doi: 10.1016/j.reprotox.2019.07.019. Epub 2019 Aug 3.
There is a growing demand for wild type mice and mouse models of disease that may be more representative of human conditions but there is little information on neonatal and juvenile mouse anatomy. This project produces sound and comprehensive histology background data on the developing neonatal mouse at different time points from Day 0 until Day 28. The work describes optimal methods for tissue harvesting, fixation and processing from the neonatal and juvenile mice which can be used in routine toxicology studies. A review of the available literature revealed inconsistencies in the developmental milestones reported in the mouse. Although it is true that the sequence of events during the development is virtually the same in mice and rats, important developmental milestones in the mouse often happen earlier than in the rat, and these species should not be used interchangeably.
目前，人们对更能代表人类疾病状况的野生型小鼠和疾病模型鼠的需求日益增长，但有关新生和幼年小鼠解剖结构的信息却很少。本项目在不同时间点（从出生 0 天到 28 天）生成了有关发育中新生小鼠的健全、全面的组织学背景数据。该工作描述了从新生和幼年小鼠中进行组织采集、固定和处理的最佳方法，可用于常规毒理学研究。对现有文献的回顾表明，文献中报道的小鼠发育里程碑存在不一致的情况。尽管实际上，小鼠和大鼠的发育过程中的事件顺序几乎相同，但在小鼠中，重要的发育里程碑往往发生得更早，因此这两种物种不能互换使用。
