Kim Dong-Gwan, Bahmani Ramin, Ko Jae-Heung, Hwang Seongbin
Department of Bioindustry and Bioresource Engineering, Department of Molecular Biology and Plant Engineering Research Institute, Sejong University, Seoul 05006, Korea.
Department of Plant & Environmental New Resources, Kyung Hee University, Kyeonggi-do 17104, Korea.
Plants (Basel). 2019 Aug 5;8(8):266. doi: 10.3390/plants8080266.
Environmental androgen analogues act as endocrine disruptors, which inhibit the normal function of androgen in animals. In the present work, through the expression of a chimeric gene specified for the production of the anthocyanin in response to androgen DHT (dihydrotestosterone), we generated an indicator that displays a red color in leaves in the presence of androgen compounds. This construct consists of a ligand-binding domain of the human androgen receptor gene and the poplar transcription factor gene , which is involved in anthocyanin biosynthesis in poplar and . The transgenic XVA- displayed a red color in leaves in response to 10 ppm DHT, whereas it did not react in the presence of other androgenic compounds. The transcript level of peaked at day 13 of DHT exposure on agar media and then declined to its normal level at day 15. Expressions of anthocyanin biosynthesis genes including chalcone flavanone isomerase, chalcone synthase, flavanone 3-hydroxylase, dihydroflavonol 4-reductase, UFGT (UGT78D2), and anthocyanidin synthase were similar to that of . It is assumed that this transgenic plant can be used by nonscientists for the detection of androgen DHT in the environment and samples such as food solution without any experimental procedures.
环境雄激素类似物作为内分泌干扰物，会抑制动物体内雄激素的正常功能。在本研究中，通过表达一种嵌合基因，该基因可响应雄激素双氢睾酮（DHT）产生花青素，我们构建了一种指示剂，在存在雄激素化合物时叶片会呈现红色。该构建体由人类雄激素受体基因的配体结合结构域和杨树转录因子基因组成，后者参与杨树中的花青素生物合成。转基因植株XVA - 在10 ppm DHT作用下叶片呈现红色，而在其他雄激素化合物存在时无反应。在琼脂培养基上，DHT处理13天时转录水平达到峰值，然后在第15天降至正常水平。包括查尔酮黄烷酮异构酶、查尔酮合酶、黄烷酮3 - 羟化酶、二氢黄酮醇4 - 还原酶、UDP - 葡萄糖基转移酶（UGT78D2）和花青素合酶在内的花青素生物合成基因的表达与该基因相似。据推测，这种转基因植物可被非专业人员用于检测环境及食物溶液等样品中的雄激素DHT，无需任何实验步骤。
