Rha Chan-Su, Jeong Hyun Woo, Park Saitbyul, Lee Siyoung, Jung Young Sung, Kim Dae-Ok
Vitalbeautie Research Division, Amorepacific Corporation R&D Center, Yongin 17074, Korea.
Safety and Regulatory Division, Amorepacific Corporation R&D Center, Yongin 17074, Korea.
Antioxidants (Basel). 2019 Aug 5;8(8):278. doi: 10.3390/antiox8080278.
(1) Background: Extensive research has focused on flavan-3-ols, but information about the bioactivities of green tea flavonols is limited. (2) Methods: In this study, we investigated the antioxidative, anti-inflammatory, and anticancer effects of flavonol glycosides and aglycones from green tea using in vitro cell models. The fractions rich in flavonol glycoside (FLG) and flavonol aglycone (FLA) were obtained from green tea extract after treatment with tannase and cellulase, respectively. (3) Results: FLG and FLA contained 16 and 13 derivatives, respectively, including apigenin, kaempferol, myricetin, and quercetin, determined by mass spectrometry. FLA exhibited higher radical-scavenging activity than that of FLG. FLG and FLA attenuated the levels of intracellular oxidative stress in neuron-like PC-12 cells. The treatment of RAW 264.7 murine macrophages with FLG and FLA significantly reduced the mRNA expression of inflammation-related genes in a dose-dependent manner. Furthermore, FLG and FLA treatments decreased the viability of the colon adenoma cell line DLD-1 and breast cancer cell line E0771. Moreover, the treatment with FLG or FLA combined with paclitaxel had synergistic anticancer effects on the DLD-1 cell line. (4) Conclusions: Flavonols from green tea exerted beneficial effects on health and may be superior to flavan-3-ols.
(1) 背景：大量研究聚焦于黄烷 - 3 - 醇，但关于绿茶黄酮醇生物活性的信息有限。(2) 方法：在本研究中，我们使用体外细胞模型研究了绿茶中黄酮醇糖苷和苷元的抗氧化、抗炎和抗癌作用。富含黄酮醇糖苷（FLG）和黄酮醇苷元（FLA）的组分分别是用单宁酶和纤维素酶处理绿茶提取物后获得的。(3) 结果：通过质谱测定，FLG和FLA分别含有16种和13种衍生物，包括芹菜素、山奈酚、杨梅素和槲皮素。FLA表现出比FLG更高的自由基清除活性。FLG和FLA降低了神经元样PC - 12细胞内的氧化应激水平。用FLG和FLA处理RAW 264.7小鼠巨噬细胞显著降低了炎症相关基因的mRNA表达，且呈剂量依赖性。此外，FLG和FLA处理降低了结肠腺癌细胞系DLD - 1和乳腺癌细胞系E0771的活力。而且，用FLG或FLA与紫杉醇联合处理对DLD - 1细胞系具有协同抗癌作用。(4) 结论：绿茶中的黄酮醇对健康有有益作用，可能优于黄烷 - 3 - 醇。
