Cho Chi Heung, Jang Holim, Lee Migi, Kang Hee, Heo Ho Jjn, Kim Dae-Ok
Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
Damtuh Co., Ltd., Seoul 06561, Republic of Korea.
J Microbiol Biotechnol. 2017 Jul 28;27(7):1257-1265. doi: 10.4014/jmb.1704.04033.
The present study was carried out to investigate the antioxidative and neuroprotective effects of sea buckthorn ( L.) leaves (SBL) harvested at different times. Reversed-phase high-performance liquid chromatography analysis revealed five major phenolic compounds: ellagic acid, gallic acid, isorhamnetin, kaempferol, and quercetin. SBL harvested in August had the highest total phenolic and flavonoid contents and antioxidant capacity. Treatment of neuronal PC-12 cells with the ethyl acetate fraction of SBL harvested in August increased their viability and membrane integrity and reduced intracellular oxidative stress in a dose-dependent manner. The relative populations of both early and late apoptotic PC-12 cells were decreased by treatment with the SBL ethyl acetate fraction, based on flow cytometry analysis using annexin V-FITC/PI staining. These findings suggest that SBL can serve as a good source of antioxidants and medicinal agents that attenuate oxidative stress.
本研究旨在调查不同时期采收的沙棘（Hippophae rhamnoides L.）叶（SBL）的抗氧化和神经保护作用。反相高效液相色谱分析显示了五种主要的酚类化合物：鞣花酸、没食子酸、异鼠李素、山奈酚和槲皮素。8月采收的SBL总酚和黄酮含量最高，抗氧化能力最强。用8月采收的SBL的乙酸乙酯部位处理神经元PC-12细胞，可提高其活力和膜完整性，并以剂量依赖的方式降低细胞内氧化应激。基于使用膜联蛋白V-FITC/PI染色的流式细胞术分析，用SBL乙酸乙酯部位处理可降低早期和晚期凋亡PC-12细胞的相对比例。这些发现表明，SBL可作为抗氧化剂和减轻氧化应激的药物的良好来源。
