a College of Biotechnology, Southwest University , Chongqing , China.
b Department of Production and Management, Tibet Autonomous Region Veterinary Biologics Factory , Lasa , Tibet , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3338-3349. doi: 10.1080/21691401.2019.1648278.
The emerging and spreading of multi-drug resistant (MDR) bacteria have been becoming one of the most severe threats to human health. Enhancing oxidative stress as mimicking immune system was considered as a potential strategy to fight against infection of MDR bacteria. In this study, we investigated the antibacterial efficiency of such a strategy which combines silver nanoparticles (AgNPs) with ebselen. The results showed that AgNPs and ebselen combination had significant synergistic killing effects both on () and () , including model strains of China Veterinary Culture Collection and MDR clinical isolates, which is similar as the combination of silver ion and ebselen. AgNPs exhibited to be a strong inhibitor of bacterial thioredoxin reductase, same as a free silver ion. Ebselen mitigated the cytotoxicity of AgNPs to HeLa cells. However, in a bacteria-cell coexistence condition, the synergistic bactericidal effect was only observed on (<.05), while the temporary synergistic inhibitory effect on within 4 hours treatment (<.01). In mice infection model, a combination of AgNPs and ebselen did not increase protection against the challenge of clinical CQ10 strain. Our data demonstrated that AgNPs and ebselen combination may be a promising strategy to fight against the increasingly MDR bacteria targeting bacterial thiol redox system.
多药耐药（MDR）细菌的出现和传播已成为对人类健康最严重的威胁之一。增强氧化应激以模拟免疫系统被认为是对抗 MDR 细菌感染的一种潜在策略。在这项研究中，我们研究了将银纳米颗粒（AgNPs）与埃布斯隆结合的这种策略的抗菌效率。结果表明，AgNPs 和埃布斯隆联合对（）和（）具有显著的协同杀伤作用，包括中国兽医文化收藏的模型菌株和 MDR 临床分离株，这与银离子和埃布斯隆的联合作用相似。AgNPs 表现出强烈抑制细菌硫氧还蛋白还原酶的作用，与游离银离子相同。埃布斯隆减轻了 AgNPs 对 HeLa 细胞的细胞毒性。然而，在细菌-细胞共存的条件下，仅在（<0.05）时观察到对（）的协同杀菌作用，而在 4 小时处理内对（）的暂时协同抑制作用（<0.01）。在小鼠感染模型中，AgNPs 和埃布斯隆的联合使用并没有增加对临床 CQ10 菌株挑战的保护作用。我们的数据表明，AgNPs 和埃布斯隆联合可能是针对细菌硫醇氧化还原系统的日益增加的 MDR 细菌的一种有前途的策略。
