Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, Colorado 80523-1177, USA; email:
IRD, CIRAD, Université Montpellier, IPME, 34000 Montpellier, France; email:
Annu Rev Phytopathol. 2019 Aug 25;57:459-481. doi: 10.1146/annurev-phyto-082718-100026. Epub 2019 Aug 6.
Transcription activator-like effectors (TALEs) from the genus are proteins with the remarkable ability to directly bind the promoters of genes in the plant host to induce their expression, which often helps bacterial colonization. Metaphorically, TALEs act as spies that infiltrate the plant disguised as high-ranking civilians (transcription factors) to trick the plant into activating weak points that allow an invasion. Current knowledge of how TALEs operate allows researchers to predict their activity (counterespionage) and exploit their function, engineering them to do our bidding (a Manchurian agent). This has been possible thanks particularly to the discovery of their DNA binding mechanism, which obeys specific amino acid-DNA correspondences (the TALE code). Here, we review the history of how researchers discovered the way these proteins work and what has changed in the ten years since the discovery of the code. Recommended music for reading this review can be found in the .
转录激活样效应因子（TALEs）来自 属，是一类具有直接结合植物宿主基因启动子以诱导其表达的非凡能力的蛋白质，这通常有助于细菌的定植。比喻地说，TALEs 像间谍一样，伪装成高级平民（转录因子）潜入植物体内，欺骗植物激活弱点，从而允许入侵。目前对 TALEs 如何发挥作用的了解使研究人员能够预测它们的活性（反间谍）并利用它们的功能，对其进行工程设计以实现我们的意图（一个满洲代理人）。这要归功于发现了它们的 DNA 结合机制，该机制遵循特定的氨基酸-DNA 对应关系（TALE 密码）。在这里，我们回顾了研究人员发现这些蛋白质工作方式的历史，以及自密码发现以来十年中发生的变化。阅读本评论的推荐音乐可在 中找到。
