Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul, 08826, South Korea.
Department of Brain and Cognitive Engineering, Korea University, Seoul, 02841, South Korea.
Biochem Biophys Res Commun. 2019 Oct 1;517(4):703-708. doi: 10.1016/j.bbrc.2019.07.119. Epub 2019 Aug 3.
Electroporation is used for cancer therapy to efficiently destroy cancer tissues by transferring anticancer drugs into cancer cells or by irreversible tumor ablation without resealing pores. There is growing interest in the electroporation method for the treatment of lung cancer, which has the highest mortality rate among cancers. Improving the cancer cell selectivity has the potential to expand its use. However, the factors that influence the cell selectivity of electroporation are debatable. We aimed to identify the important factors that influence the efficiency of electroporation in lung cells. The electropermeabilization of lung cancer cells (H460, A549, and HCC1588) and normal lung cells (MRC5, WI26 and L132) was evaluated by the transfer of fluorescence dyes. We found that membrane permeabilization increased as cell size, membrane stiffness, resting transmembrane potential, and lipid cholesterol ratio increased. Among them, lipid composition was found to be the most relevant factor in the electroporation of lung cells. Our results provide insight into the differences between lung cancer cells and normal lung cells and provide a basis for enhancing the sensitivity of lung cancers cells to electroporation.
电穿孔被用于癌症治疗，通过将抗癌药物转导到癌细胞中或通过不可逆的肿瘤消融而不重新封闭孔来有效地破坏癌症组织。电穿孔方法在治疗肺癌方面的应用越来越受到关注，肺癌是癌症中死亡率最高的一种。提高癌细胞的选择性有可能扩大其应用。然而，影响电穿孔细胞选择性的因素仍存在争议。我们旨在确定影响肺癌细胞电穿孔效率的重要因素。通过荧光染料的转导来评估肺癌细胞（H460、A549 和 HCC1588）和正常肺细胞（MRC5、WI26 和 L132）的电穿孔通透性。我们发现，随着细胞大小、膜硬度、静息跨膜电位和脂质胆固醇比例的增加，膜通透性增加。其中，脂质组成被发现是肺细胞电穿孔中最相关的因素。我们的结果提供了对肺癌细胞和正常肺细胞之间差异的深入了解，并为提高肺癌细胞对电穿孔的敏感性提供了依据。
