生长过程中的温度条件对通过电穿孔获得的转化频率的影响。
The effect of temperature conditions during growth on the transformation frequency of obtained by electroporation.
作者信息
Kania Kinga, Levytska Anna, Drożak Anna, Andrzej Borkowski, Paweł Działak, Zienkiewicz Maksymilian
机构信息
Department of Molecular Plant Physiology, University of Warsaw, Warsaw, Poland.
Faculty of Geology, Geophysics and Environmental Protection, AGH University of Science and Technology, Krakow, Poland.
出版信息
Biochem Biophys Rep. 2022 Feb 7;30:101220. doi: 10.1016/j.bbrep.2022.101220. eCollection 2022 Jul.
Abstract
In this study, we have shown that transformation efficiency of C-169 obtained by electroporation can be significantly increased by either supra- or sub-optimal growth temperatures.
摘要
在本研究中，我们已经表明，通过超最佳或次最佳生长温度，电穿孔获得的C-169的转化效率可显著提高。
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