Department of Pathology and Pathophysiology, Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, Hubei, China.
Department of Anatomy, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, Hubei, China.
Pathol Res Pract. 2019 Oct;215(10):152575. doi: 10.1016/j.prp.2019.152575. Epub 2019 Aug 1.
The important role of LncRNA in the development of breast cancer is attracting more and more attention. In the previous study, we found that the expression level of LncRNA SNHG6 in breast cancer tissues and cells was significantly increased, but its mechanism in the development of breast cancer was still unclear. Our study found that knockdown of SNHG6 significantly inhibited the proliferation, migration and invasion of breast cancer cells MCF-7 and MDA-MB-231 cells. Further study showed that knockdown of SNHG6 significantly inhibited the expression level of VASP. More importantly, SNHG6 and VASP both can bind directly to miR-26a, suggesting that SNHG6 could act as a ceRNA to sponge miR-26a, thereby promoting the expression of VASP, which leading to activated proliferation, migration and invasion of breast cancer cells. Taken together, this study revealed the important role of the SNHG6/miR-26a/VASP regulatory network in the development of breast cancer, and provided a reference for exploring new pathogenesis and biomarkers of breast cancer.
LncRNA 在乳腺癌发生发展中的重要作用正越来越受到关注。在之前的研究中，我们发现 LncRNA SNHG6 在乳腺癌组织和细胞中的表达水平显著升高，但它在乳腺癌发生发展中的作用机制尚不清楚。我们的研究发现，敲低 SNHG6 可显著抑制乳腺癌细胞 MCF-7 和 MDA-MB-231 细胞的增殖、迁移和侵袭。进一步的研究表明，敲低 SNHG6 可显著抑制 VASP 的表达水平。更重要的是，SNHG6 和 VASP 均可直接与 miR-26a 结合，提示 SNHG6 可作为 ceRNA 来海绵吸附 miR-26a，从而促进 VASP 的表达，进而激活乳腺癌细胞的增殖、迁移和侵袭。综上所述，本研究揭示了 SNHG6/miR-26a/VASP 调控网络在乳腺癌发生发展中的重要作用，为探索乳腺癌新的发病机制和生物标志物提供了参考。
