Suprasert A, Fujioka T
Department of Veterinary Anatomy, Faculty of Agriculture, Nagoya University, Japan.
Acta Histochem. 1988;83(2):141-51. doi: 10.1016/S0065-1281(88)80047-3.
In the cecal epithelium of the chicken, glycoconjugates were studied by light microscopy with lectin-peroxidase and correlated procedures, and by electron microscopy with high iron diamine-thiocarbohydrazide-silver proteinate (HID-TCH-SP) and periodic acid (PA)-TCH-SP methods. Mucous granules of goblet cells and striated border of columnar cells at the basal cecum contained acidic and neutral glycoconjugates with sulfate ester, vicinal diol groupings and alpha-D-mannose, alpha-D-glucose, beta-D-galactose, N-acetyl-D-glucosamine, and sialic acid-galactose dimers. In addition, terminal galactose-(1-3)N-acetylgalactosamine disaccharides were detected. In the apical cecum, similar glycoconjugates involved in the mucous granules of goblet cells and the striated border of columnar cells were found to be smaller in amount. Terminal galactose-(1-3)N-acetylgalactosamine disaccharides together with glycogen particles were also visualized in columnar cells of the basal and apical cecum. The histophysiological significance of glycoconjugates involved in the chicken cecal epithelium was discussed.
在鸡的盲肠上皮中，通过凝集素-过氧化物酶及相关程序进行光学显微镜研究，并采用高铁二胺-硫代碳酰肼-银蛋白（HID-TCH-SP）和高碘酸（PA）-TCH-SP方法进行电子显微镜研究，对糖缀合物进行了研究。盲肠基部杯状细胞的黏液颗粒和柱状细胞的纹状缘含有酸性和中性糖缀合物，其带有硫酸酯、邻二醇基团以及α-D-甘露糖、α-D-葡萄糖、β-D-半乳糖、N-乙酰-D-葡糖胺和唾液酸-半乳糖二聚体。此外，还检测到末端半乳糖-(1-3)N-乙酰半乳糖胺二糖。在盲肠顶部，发现杯状细胞黏液颗粒和柱状细胞纹状缘中涉及的类似糖缀合物数量较少。末端半乳糖-(1-3)N-乙酰半乳糖胺二糖以及糖原颗粒也在盲肠基部和顶部的柱状细胞中可见。文中讨论了鸡盲肠上皮中糖缀合物的组织生理意义。
