Suprasert A, Fujioka T
Department of Veterinary Anatomy, Faculty of Agriculture, Nagoya University, Japan.
Anat Anz. 1990;170(2):91-8.
The lectin binding pattern in the mucous epithelium of the chicken proventriculus was studied by using a peroxidase labeling method and correlated procedure. The nature of glycoconjugates in the mucous epithelium was found to change during the upward cell migration from deep to superficial parts. The superficial mucous epithelium (plica) was found to contain predominantly neutral glycoconjugates with N-acetyl-D-glucosamine residues and terminal galactose-N-acetylgalactosamine disaccharides whereas the deep epithelium (sulcus) contained acidic sulfated glycoconjugates with terminal sialic acid-galactose dimer. A continuous intraluminal mucous layer over the surface epithelium was found to contain a mixture of glycoconjugates secreted from both the plica and sulcus mucous cells. The data suggests that the changes in glycoconjugates of epithelial cells were closely correlated with cellular maturation.
采用过氧化物酶标记法及相关程序，研究了鸡前胃黏膜上皮中的凝集素结合模式。结果发现，在黏液上皮细胞从深部向浅部向上迁移的过程中，糖缀合物的性质发生了变化。浅表黏液上皮（皱襞）主要含有带有N-乙酰-D-葡萄糖胺残基和末端半乳糖-N-乙酰半乳糖胺二糖的中性糖缀合物，而深部上皮（沟）则含有带有末端唾液酸-半乳糖二聚体的酸性硫酸化糖缀合物。在表面上皮上发现的连续腔内黏液层含有从皱襞和沟黏液细胞分泌的糖缀合物混合物。数据表明，上皮细胞糖缀合物的变化与细胞成熟密切相关。
