Lidstrom M E
Keck Laboratories, California Institute of Technology, Pasadena 91125.
Antonie Van Leeuwenhoek. 1988;54(3):189-99. doi: 10.1007/BF00443577.
Four new methane-oxidizing bacteria have been isolated from marine samples taken at the Hyperion sewage outfall, near Los Angeles, CA. These bacteria require NaCl for growth. All exhibit characteristics typical of Type I methanotrophs, except they contain enzyme activities of both the ribulose monophosphate pathway and the serine cycle. All four strains are characterized by rapid growth in liquid culture and on agar plates, and all have temperature optima above 35 degrees C. One strain, chosen for further study, has been shown to maintain broadhost range cloning vectors and is currently being used for genetic studies.
从加利福尼亚州洛杉矶附近海波龙污水排放口采集的海洋样本中分离出了四种新的甲烷氧化菌。这些细菌生长需要氯化钠。所有菌株均表现出典型的I型甲烷营养菌的特征，只是它们同时具有磷酸核酮糖途径和丝氨酸循环的酶活性。所有四个菌株的特点是在液体培养物和琼脂平板上生长迅速，并且所有菌株的最适温度均高于35摄氏度。选择了其中一个菌株进行进一步研究，已证明它能维持广泛宿主范围的克隆载体，目前正用于遗传学研究。
