Lhullier C, Moritz M I G, Tabalipa E O, Sardá F N, Schneider N F Z, Moraes M H, Constantino L, Reginatto F H, Steindel M, Pinheiro U S, Simões C M O, Pérez C D, Schenkel E P
Laboratório de Produtos Naturais, Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina - UFSC, Rua Eng. Agronômico Andrei Cristian Ferreira, s/n, Campus Trindade, CEP 88040-900, Florianópolis, SC, Brasil.
Laboratório de Virologia Aplicada, Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina - UFSC, Rua Eng. Agronômico Andrei Cristian Ferreira, s/n, Campus Trindade, CEP 88040-900, Florianópolis, SC, Brasil.
Braz J Biol. 2020 Apr-Jun;80(2):393-404. doi: 10.1590/1519-6984.213678.
This paper reports the in vitro antiproliferative effects, antiprotozoal, anti-herpes and antimicrobial activities of 32 organic extracts of 14 marine sponges and 14 corals collected in northeast Brazilian coast. The ethanolic extracts of the sponges Amphimedon compressa and Tedania ignis, and the acetone extract of Dysidea sp. showed relevant results concerning the antiproliferative effects against A549, HCT-8, and PC-3 cell lines by sulforhodamine B assay, but also low specificity. Concerning the antiprotozoal screening, the ethanolic extract of Amphimedon compressa and the acetone and ethanolic extracts of Dysidea sp. were the most active against Leishmania amazonensis and Trypanosoma cruzi expressing β-galactosidase in THP-1 cells. In the preliminary anti-HSV-1 (KOS strain) screening, the ethanolic extracts of the sponges Amphimedon compressa, Haliclona sp. and Chondrosia collectrix inhibited viral replication by more than 50%. The most promising anti-herpes results were observed for the ethanolic extract of Haliclona sp. showing high selective indices against HSV-1, KOS and 29R strains (SI> 50 and >79, respectively), and HSV-2, 333 strain (IS>108). The results of the antibacterial screening indicated that only the ethanolic extract of Amphimedon compressa exhibited a weak activity against Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli by the disk diffusion method. In view of these results, the extracts of Amphimedon compressa, Tedania ignis and Dysidea sp. were selected for further studies aiming the isolation and identification of the bioactive compounds with antiproliferative and/or antiprotozoal activities. The relevant anti-herpes activity of the ethanolic extract of Haliclona sp. also deserves special attention, and will be further investigated.
本文报道了从巴西东北海岸采集的14种海洋海绵和14种珊瑚的32种有机提取物的体外抗增殖作用、抗原虫、抗疱疹和抗菌活性。海绵压缩艾氏海绵（Amphimedon compressa）和火红特氏海绵（Tedania ignis）的乙醇提取物，以及皮海绵属（Dysidea sp.）的丙酮提取物，通过磺酰罗丹明B法对A549、HCT-8和PC-3细胞系显示出相关的抗增殖作用结果，但特异性也较低。关于抗原虫筛选，压缩艾氏海绵的乙醇提取物以及皮海绵属的丙酮和乙醇提取物对在THP-1细胞中表达β-半乳糖苷酶的亚马逊利什曼原虫（Leishmania amazonensis）和克氏锥虫（Trypanosoma cruzi）活性最强。在初步抗单纯疱疹病毒1型（KOS株）筛选中，压缩艾氏海绵、Haliclona sp.和聚集软骨海绵（Chondrosia collectrix）的乙醇提取物抑制病毒复制超过50%。对Haliclona sp.的乙醇提取物观察到最有前景的抗疱疹结果，其对单纯疱疹病毒1型KOS株和29R株显示出高选择性指数（分别为SI>50和>79），对单纯疱疹病毒2型333株显示出高选择性指数（SI>108）。抗菌筛选结果表明，只有压缩艾氏海绵的乙醇提取物通过纸片扩散法对粪肠球菌（Enterococcus faecalis）、铜绿假单胞菌（Pseudomonas aeruginosa）和大肠杆菌（Escherichia coli）表现出微弱活性。鉴于这些结果，选择压缩艾氏海绵、火红特氏海绵和皮海绵属的提取物进行进一步研究，旨在分离和鉴定具有抗增殖和/或抗原虫活性的生物活性化合物。Haliclona sp.乙醇提取物相关的抗疱疹活性也值得特别关注，并将进一步研究。
