Jacquot J, Goldstein G, Sommerhoff C, Benali R, Puchelle E, Basbaum C B
INSERM U 314, Université de Reims, France.
Biochem Biophys Res Commun. 1988 Sep 15;155(2):857-62. doi: 10.1016/s0006-291x(88)80574-6.
Bovine tracheal submucosal gland serous cells were incubated with (35S) methionine. The proteins synthesized and secreted into the culture medium during pulse and chase periods were analyzed. Three major protein bands with apparent molecular weight values of 67 kD, 47 kD and 32 kD were detected by fluorography following SDS-polyacrylamide gel electrophoresis. Based on the molecular weight, immuno-reactive cross reactivity and surface molecular change characteristics, the labeled Mr 67 kD protein was identified as an albumin-like protein. Our results suggest that albumin found in airway secretions can originate, at least in part, from tracheal gland serous cells.
将牛气管黏膜下腺浆液性细胞与（35S）甲硫氨酸一起孵育。分析了在脉冲和追踪期合成并分泌到培养基中的蛋白质。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后，通过荧光自显影检测到三条主要蛋白带，其表观分子量值分别为67 kD、47 kD和32 kD。根据分子量、免疫反应交叉反应性和表面分子变化特征，将标记的67 kD蛋白鉴定为类白蛋白样蛋白。我们的结果表明，气道分泌物中发现的白蛋白至少部分可源自气管腺浆液性细胞。
