Jacquot J, Dupuit F, Elbtaouri H, Hinnrasky J, Antonicelli F, Haye B, Puchelle E
INSERM U314, Université de Reims, CHU Maison Blanche, France.
FEBS Lett. 1990 Nov 12;274(1-2):131-5. doi: 10.1016/0014-5793(90)81347-q.
Evidence is obtained for the presence of lipocortin-like proteins in human tracheal gland cells in culture. Using polyclonal antibodies to lipocortin I, indirect immunofluorescence studies demonstrate that lipocortin I is mainly confined to the tracheal gland cell surface. From cell membranes, four Ca2(+)-dependent proteins (35, 40, 45 and 67 kDa) were identified as lipocortin related proteins by using immunoblotting and fluorography following [35S]methionine metabolic labeling experiments. A strong immunoreactivity for the 35 kDa protein was observed. In addition, lipocortin-like proteins with apparent Mr33, 35, 37 and 67 kDa, respectively, were released in the apical culture medium by tracheal gland cells cultured on microporous membrane of a double chamber culture system.
有证据表明，在体外培养的人气管腺细胞中存在脂皮质素样蛋白。利用针对脂皮质素I的多克隆抗体，间接免疫荧光研究表明，脂皮质素I主要局限于气管腺细胞表面。在细胞膜上，通过[35S]甲硫氨酸代谢标记实验后的免疫印迹和荧光自显影，鉴定出四种钙依赖性蛋白（35、40、45和67 kDa）为脂皮质素相关蛋白。观察到35 kDa蛋白有强烈的免疫反应性。此外，在双室培养系统微孔膜上培养的气管腺细胞将表观分子量分别为33、35、37和67 kDa的脂皮质素样蛋白释放到顶端培养基中。
