Shliapnikov S V, Bezborodova S I, Dement'ev A A, Kulikov V A
Bioorg Khim. 1988 May;14(5):589-95.
The complete amino acid sequence of an extracellular guanyl-specific RNase from Aspergillus pallidus fungi has been established. The RNase contains 104 amino acid residues (Mr 11,029). Its primary structure was analyzed basing on the automated Edman degradation of the carboxymethylated RNase followed by tryptic digestion and sequencing of the resultant hydrolysate. An additional structural information was obtained by means of the automatic sequencing of the cyanogen bromide peptide mixture and by studying the kinetics of the RNase's digestion with carboxypeptidase Y.
已确定来自苍白曲霉真菌的一种细胞外鸟苷特异性核糖核酸酶的完整氨基酸序列。该核糖核酸酶含有104个氨基酸残基（分子量11,029）。其一级结构是基于羧甲基化核糖核酸酶的自动埃德曼降解，随后进行胰蛋白酶消化并对所得水解产物进行测序来分析的。通过对溴化氰肽混合物的自动测序以及研究核糖核酸酶用羧肽酶Y消化的动力学，获得了额外的结构信息。
