来自真菌粗糙脉孢菌的鸟嘌呤特异性核糖核酸酶N1的氨基酸序列。
The amino acid sequence of ribonuclease N1, a guanine-specific ribonuclease from the fungus Neurospora crassa.
作者信息
Takahashi K
机构信息
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo.
出版信息
J Biochem. 1988 Sep;104(3):375-82. doi: 10.1093/oxfordjournals.jbchem.a122476.
The complete amino acid sequence of ribonuclease N1 (RNase N1), a guanine-specific ribonuclease from a fungus, Neurospora crassa, was determined by conventional protein sequencing, using peptide fragments obtained by tryptic digestion of cyanogen bromide-treated RNase N1 and by Staphylococcus aureus V8 protease digestion of heat-denatured RNase N1. The results showed that the protein is composed of a single polypeptide chain of 104 amino acid residues cross-linked by two disulfide bonds and has a molecular weight of 11,174: (sequence; see text) (Disulfide bonds: C2-C10, C6-C103) The amino acid sequence was homologous with those of RNase T1 (65% identity) and related microbial RNases.
核糖核酸酶N1（RNase N1）是一种来自真菌粗糙脉孢菌的鸟嘌呤特异性核糖核酸酶，其完整氨基酸序列通过传统蛋白质测序法确定。该方法使用了经溴化氰处理的RNase N1经胰蛋白酶消化得到的肽片段，以及经热变性的RNase N1经金黄色葡萄球菌V8蛋白酶消化得到的肽片段。结果表明，该蛋白质由一条104个氨基酸残基的单多肽链组成，通过两个二硫键交联，分子量为11,174：（序列；见正文）（二硫键：C2-C10，C6-C103）该氨基酸序列与RNase T1的序列同源（同一性为65%），并且与相关的微生物核糖核酸酶序列同源。
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