Larvicidal and adulticidal effects and ultrastructural changes of larvae midgut epithelium of Musca domestica (Diptera: Muscidae) fed with Bacillus thuringiensis var. kyushuensis.

作者信息

Pereira Lorrane de Andrade, Ferreira Vitor Dos Santos Baia, Leite Nahara de Souza, Souza Sandra Maria de Oliveira, Queiroz Margareth Maria de Carvalho, Côrte-Real Suzana, Zahner Viviane

机构信息

Programa de Pós-Graduação em Biodiversidade e Saúde, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brasil.

Laboratório de Entomologia Médica e Forense, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brasil.

出版信息

Rev Soc Bras Med Trop. 2019 Aug 1;52:e20190135. doi: 10.1590/0037-8682-0135-2019.

INTRODUCTION

Musca domestica is resistant to many insecticides; hence, biological control is a suitable alternative.

METHODS

We evaluated the lethality of strain Btk176 towards the larval and adult M. domestica and the histopathological effects in the larvae midgut.

RESULTS

We observed 99% larval and 78.9% adult mortality within 48 hours of spore ingestion (dosage, 2.4×108 CFU/ml). The histopathological effects were consistent with cytotoxicity. PCR analysis showed the presence of the cry1Ba gene. Transmission electron microscopy revealed a bipyramidal parasporal body. Thurigiensin activity was not detected.

CONCLUSIONS

The serovar, Btk176 might be a potential biocontrol agent for houseflies.