苏云金芽孢杆菌 H14 血清型（亚种 israelensis）δ内毒素对家蝇幼虫的影响研究。
A study of the effect of Bacillus thuringiensis serotype H14 (subspecies israelensis) delta endotoxin on Musca larva.
出版信息
Turk J Med Sci. 2015;45(4):794-9. doi: 10.3906/sag-1406-91.
BACKGROUND/AIM: House flies (Musca domestica) are of major public health concern in areas with poor sanitation and hygiene conditions. Biological control through the use of parasitoids and pathogens is one of the alternatives to the use of chemical pesticides for control of insects of public health importance.
MATERIALS AND METHODS
The effects of the delta endotoxin of Bacillus thuringiensis on house fly larval mortality were studied. Gel filtration and SDS-PAGE methods were used for separation and purification of proteins. Delta endotoxin was incubated with larvae in concentrations of 0.43 mg/mL and 0.27 mg/mL in bioassay tests.
RESULTS
The results of this study indicated protein crystal toxicity against larvae of the house fly. A concentration of 0.43 mg/mL of this toxin caused 100% mortality in house fly larvae. The LD50 amount of these toxins was calculated as 125 µg/g.
CONCLUSION
The results of this study suggest that the use of the protein crystal including delta endotoxin of Bacillus thuringiensis serotype H14 is an effective weapon in the biological fight against the house fly.
背景/目的：家蝇（Musca domestica）在卫生和卫生条件差的地区对公共卫生构成重大威胁。通过利用寄生蜂和病原体进行生物防治，是替代使用化学农药来防治对公共卫生具有重要意义的昆虫的一种选择。
材料和方法
研究了苏云金芽孢杆菌 δ 内毒素对家蝇幼虫死亡率的影响。使用凝胶过滤和 SDS-PAGE 方法进行蛋白质的分离和纯化。在家蝇幼虫生物测定试验中，以 0.43mg/mL 和 0.27mg/mL 的浓度孵育 δ 内毒素。
结果
本研究结果表明，该蛋白晶体对家蝇幼虫具有毒性。该毒素浓度为 0.43mg/mL 时，可使家蝇幼虫 100%死亡。这些毒素的 LD50 量计算为 125µg/g。
结论
本研究结果表明，使用包括苏云金芽孢杆菌 H14 血清型 δ 内毒素的蛋白晶体是对抗家蝇的生物战中的有效武器。
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