Germline Stem Cells and Microenvironment Lab, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing Jiangsu 210095, China.
College of Life Sciences, Jilin University, Changchun Jilin 130012, China.
Zool Res. 2019 Sep 18;40(5):343-348. doi: 10.24272/j.issn.2095-8137.2019.051.
Research on culture and gene editing of domestic spermatogonial stem cells (SSCs) is of considerable interest but remains a challenging issue in animal science. In recent years, some progress on the isolation, purification, and genetic manipulation of porcine SSCs has been reported. Here, we summarize the characteristics of porcine SSCs as well current advances in their culture, potential usage, and genetic manipulation. Furthermore, we discuss the current application of gene editing in pig cloning technology. Collectively, this commentary aims to summarize the progress made and obstacles encountered in porcine SSC research to better serve animal husbandry, improve livestock fecundity, and enhance potential clinical use.
关于国内精原干细胞（SSCs）的培养和基因编辑的研究具有相当的兴趣，但在动物科学领域仍然是一个具有挑战性的问题。近年来，已经有一些关于猪 SSCs 的分离、纯化和遗传操作的进展的报道。在这里，我们总结了猪 SSCs 的特性以及它们的培养、潜在用途和遗传操作的最新进展。此外，我们还讨论了基因编辑在猪克隆技术中的应用。总的来说，本篇评论旨在总结猪 SSC 研究中取得的进展和遇到的障碍，以更好地为畜牧业服务，提高家畜的繁殖力，并增强潜在的临床应用。
