Wang Xingwang, Shi Junsong, Cai Gengyuan, Zheng Enqin, Liu Dewu, Wu Zhenfang, Li Zicong
National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.
Cell Reprogram. 2019 Oct;21(5):221-228. doi: 10.1089/cell.2019.0008. Epub 2019 Aug 8.
Methyl-CpG-binding domain protein 3 () is a core component of the nucleosome remodeling and deacetylase (NuRD) complex, which is crucial for pluripotent stem cell differentiation and embryonic development. was shown to play important roles in transcription factor-induced somatic cell reprogramming. Expression level of was demonstrated to be higher in somatic cell nuclear transfer-generated cloned pig embryos than in fertilization-derived porcine embryos. However, the functions of in nuclear transfer-mediated somatic cell reprogramming are unknown. In this study, was overexpressed in cloned pig embryos, and the effects of overexpression on gene transcription, DNA methylation, and developmental competence of cloned pig embryos were analyzed. Results indicated that overexpression of in cloned pig embryos not only increased blastocyst rate and number of cells per blastocyst but also upregulated mRNA expression levels and decreased the DNA methylation of , , and genes to the levels close to those in fertilization-produced pig embryos. These findings suggest that overexpression of improves reprogramming of cloned pig embryos.
甲基化CpG结合结构域蛋白3（）是核小体重塑与去乙酰化酶（NuRD）复合体的核心组分，该复合体对多能干细胞分化和胚胎发育至关重要。已证明在转录因子诱导的体细胞重编程中发挥重要作用。研究表明，体细胞核移植产生的克隆猪胚胎中 的表达水平高于受精来源的猪胚胎。然而， 在核移植介导的体细胞重编程中的功能尚不清楚。在本研究中， 在克隆猪胚胎中过表达，并分析了 过表达对克隆猪胚胎的基因转录、DNA甲基化和发育能力的影响。结果表明， 在克隆猪胚胎中的过表达不仅提高了囊胚率和每个囊胚的细胞数，还上调了 、 和 基因的mRNA表达水平，并将其DNA甲基化降低至接近受精产生的猪胚胎中的水平。这些发现表明， 的过表达改善了克隆猪胚胎的重编程。
