Federal State Budgetary Scientific Institution Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott, Mendeleevskya line, 199034, Saint-Petersburg, Russian Federation.
Federal State Budgetary Scientific Institution Research Institute of Experimental Medicine, Russian Federation.
Curr Mol Med. 2020;20(3):202-219. doi: 10.2174/1566524019666190808103227.
Maternal natural killer cells (NK cells) are a prevailing leukocyte population in the uteroplacental bed. Current descriptions of the effect of cytokines from the placental microenvironment on the expression of receptors by trophoblast and NK cells are inadequate and contradictory. There is insufficient information about the ability of NK cells to migrate through trophoblast cells.
To assess the impact of conditioned media obtained during culturing of placentas from the first and the third trimesters of healthy pregnancies on the phenotype of trophoblast and NK cells and impact on adhesion and transmigration of NK cells through trophoblast cell layer.
We established that conditioned media obtained from both first and third trimester placentas increased the intensity of CD106, CD49e, CD49a, CD31, CD51/61, and integrin β6 expression by trophoblast cells. Conditioned media obtained from first trimester placentas increased the intensity of CD11a, CD29, CD49d, CD58, CD29 expression by NK cells. The presence of conditioned media from third trimester placentas resulted in more intense CD29, CD49d, CD11a, CD29, CD49d, and CD58 expression by NK cells. Migration of NK cells through trophoblast cells in the presence of conditioned media from first trimester placentas was increased compared with the migration level in the presence of conditioned media from third trimester placentas. This may be associated with increased expression of CD18 by NK cells.
First trimester placental secretory products increase adhesion receptor expression by both trophoblast and NK cells. Under these conditions, trophoblast is capable of ensuring NK cell adhesion and transmigration.
母体自然杀伤细胞（NK 细胞）是胎盘床中主要的白细胞群体。目前对于胎盘微环境中的细胞因子对滋养层和 NK 细胞受体表达的影响的描述还不充分且存在矛盾。关于 NK 细胞穿过滋养层细胞迁移的能力的信息还不够充分。
评估来自健康妊娠第一和第三孕期胎盘培养物的条件培养基对滋养层和 NK 细胞表型的影响，以及对 NK 细胞穿过滋养层细胞层的黏附和迁移的影响。
我们发现，来自第一和第三孕期胎盘的条件培养基均增加了滋养层细胞 CD106、CD49e、CD49a、CD31、CD51/61 和整合素 β6 的表达强度。来自第一孕期胎盘的条件培养基增加了 NK 细胞 CD11a、CD29、CD49d、CD58、CD29 的表达强度。而来自第三孕期胎盘的条件培养基则导致 NK 细胞 CD29、CD49d、CD11a、CD29、CD49d 和 CD58 的表达更为强烈。与存在第三孕期胎盘条件培养基时的迁移水平相比，存在第一孕期胎盘条件培养基时 NK 细胞穿过滋养层细胞的迁移增加。这可能与 NK 细胞 CD18 的表达增加有关。
第一孕期胎盘分泌产物增加了滋养层和 NK 细胞黏附受体的表达。在这些条件下，滋养层能够确保 NK 细胞的黏附和迁移。
