Goto M, Horiuchi Y, Okumura K, Tada T, Kawata M, Ohmori K
J Clin Invest. 1979 Sep;64(3):695-9. doi: 10.1172/JCI109511.
Two T lymphocyte-specific antisera, i.e. naturally-occurring auto-antibody to T cells of systemic lupus erythematosus patients (natural T cell toxic autoantibody) and heterologous antiserum against human brain tissue (antibrain-associated T-cell antigen), were used to detect cell surface antigens of human peripheral T lymphocytes. Nylon column-purified T cells from normal aged individuals and patients with Werner's syndrome (a premature aging syndrome) were reacted with these auto- and heterologous antibodies followed by staining with appropriate fluorescence reagents. The cells were subjected to the automated analysis with fluorescence-activated cell sorter. Fluorescence profiles to T cells of both aged individuals of over 90 yr and Werner's syndrome showed a very similar pattern, with a drastic decrease in the population that had high fluorescence intensity stained with either antiserum accompanied by the relative increase in the cell population that had low fluorescence intensity. Natural T cell toxic autoantibody comparable to that detected in systemic lupus erythematosus patients was found in the serum of six out of seven patients with Werner's syndrome, whereas normal aged individuals produced no such an autoantibody. The results suggest that Werner's syndrome has a change in the lymphocyte population very similar to old individuals, and that such a change is caused by the production of autoantibodies reactive to T lymphocytes.
两种T淋巴细胞特异性抗血清，即系统性红斑狼疮患者T细胞的天然自身抗体（天然T细胞毒性自身抗体）和抗人脑组织的异种抗血清（抗脑相关T细胞抗原），用于检测人外周血T淋巴细胞的细胞表面抗原。将来自正常老年人和沃纳综合征（一种早衰综合征）患者的尼龙柱纯化T细胞与这些自身抗体和异种抗体反应，然后用适当的荧光试剂染色。使用荧光激活细胞分选仪对细胞进行自动分析。90岁以上老年人和沃纳综合征患者的T细胞荧光图谱显示出非常相似的模式，用任何一种抗血清染色的高荧光强度细胞群体急剧减少，同时低荧光强度细胞群体相对增加。在7例沃纳综合征患者中的6例血清中发现了与系统性红斑狼疮患者中检测到的类似的天然T细胞毒性自身抗体，而正常老年人则不产生这种自身抗体。结果表明，沃纳综合征患者的淋巴细胞群体变化与老年人非常相似，并且这种变化是由对T淋巴细胞有反应的自身抗体产生所致。
