Institute of Structural and Molecular Biology, Department of Biological Sciences, Birkbeck College, University of London, Malet Street, London WC1E 7HX, United Kingdom.
Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne 1015, Switzerland.
Curr Opin Struct Biol. 2019 Oct;58:105-114. doi: 10.1016/j.sbi.2019.05.024. Epub 2019 Aug 5.
Recent advances in structure determination of sub-cellular structures using cryo-electron microscopy and tomography have enabled us to understand their architecture in a more detailed manner and gain insight into their function. The choice of approach to use for atomic model building, fitting, refinement and validation in the 3D map resulting from these experiments depends primarily on the resolution of the map and the prior information on the corresponding model. Here, we survey some of such methods and approaches and highlight their uses in specific recent examples.
近年来，利用冷冻电子显微镜和断层扫描技术对亚细胞结构进行结构测定方面的进展，使我们能够以更详细的方式了解它们的结构，并深入了解它们的功能。在这些实验得到的 3D 图谱中，用于原子模型构建、拟合、精修和验证的方法的选择主要取决于图谱的分辨率和相应模型的先验信息。在这里，我们调查了其中的一些方法和方法，并强调了它们在最近的特定示例中的应用。
