Ahmed Farid E
Department of Radiation Oncology, Leo W. Jenkins Cancer Center, The Brody School of Medicine at East Carolina University, Greenville, NC, U.S.A.
Cancer Genomics Proteomics. 2005 Nov-Dec;2(6):317-332. Epub 2005 Nov 1.
Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) has simplified and enhanced the quantification of gene expression. However, since no agreed standardizations are available, care must be exercised when designing experiments, including the choice of appropriate amplification primers, detection chemistry and the normalization procedure, in order to obtain meaningful results. Coupling quantitative polymerase chain reaction (qPCR) to cell purification from tumor tissue has made it possible to decrease the variability in expression from in vivo heterogeneous cell populations. Sensitive and specific qRT-PCR has advanced the diagnosis, prognosis and prediction response of colorectal cancer to therapy.
定量实时逆转录聚合酶链反应（RT-PCR）简化并增强了基因表达的定量分析。然而，由于目前尚无统一的标准化方法，在设计实验时必须谨慎，包括选择合适的扩增引物、检测化学方法和标准化程序，以获得有意义的结果。将定量聚合酶链反应（qPCR）与从肿瘤组织中纯化细胞相结合，使得降低体内异质细胞群体表达的变异性成为可能。灵敏且特异的qRT-PCR推动了结直肠癌的诊断、预后评估及对治疗反应的预测。
