多头绦虫抗原B和硫氧还蛋白过氧化物酶的表达及血清学诊断潜力
Expression and serodiagnostic potential of antigen B and thioredoxin peroxidase from Taenia multiceps.
作者信息
Liu Yuchen, Yang Yingdong, Xu Jing, Dong Xiaowei, Gu Xiaobin, Xie Yue, Lai Weimin, Jing Bo, Peng Xuerong, Yang Guangyou
机构信息
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China.
Panzhihua Academy of Agricultural and Forestry Sciences, Panzhihua 617000, China.
出版信息
Vet Parasitol. 2019 Aug;272:58-63. doi: 10.1016/j.vetpar.2019.07.002. Epub 2019 Jul 12.
Coenurosis is a serious parasitic disease of herbivorous animals caused by the metacestode of Taenia multiceps (Coenurus cerebralis). Accordingly, a significant amount of research is currently dedicated to the development of appropriate antigens for use in rapid and accurate coenurosis diagnosis kits. In the present study, antigen B (AgB) and thioredoxin peroxidase (TPx) from T. multiceps were cloned and expressed using a prokaryotic system, molecular characterization of Tm-AgB was determined by bioinformatical analyses. The serological diagnostic potentials of rTm-AgB and rTm-TPx were evaluated by indirect ELISA and compared with those of previously reported rTm-AnxB2, rTm-HSP70, and rTm-GST. The results showed that Tm-AgB is a specific lipoprotein of cestodes with good thermal stability. The ELISA assay showed that rTm-AgB exhibited a sensitivity of 95.8% and a specificity of 87.5%, indicating its strong potential for serological diagnosis of T. multiceps.
多头蚴病是由多头绦虫（脑多头蚴）的中绦期幼虫引起的草食动物的一种严重寄生虫病。因此，目前大量研究致力于开发适用于快速准确的多头蚴病诊断试剂盒的抗原。在本研究中，使用原核系统克隆并表达了多头绦虫的抗原B（AgB）和硫氧还蛋白过氧化物酶（TPx），通过生物信息学分析确定了Tm-AgB的分子特征。通过间接ELISA评估了rTm-AgB和rTm-TPx的血清学诊断潜力，并与先前报道的rTm-AnxB2、rTm-HSP70和rTm-GST的潜力进行了比较。结果表明，Tm-AgB是一种具有良好热稳定性的绦虫特异性脂蛋白。ELISA检测显示，rTm-AgB的敏感性为95.8%，特异性为87.5%，表明其在多头绦虫血清学诊断中具有强大潜力。
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