中国多头绦虫的分子特征及系统发育分析
Molecular characterization and phylogenetic analysis of Taenia multiceps from China.
作者信息
Tan L, Wang A B, Zheng S Q, Zhang X L, Huang C J, Liu W
机构信息
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province 410128, PR China.
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Changsha, Hunan Province 410128, PR China.
出版信息
Acta Parasitol. 2018 Dec 19;63(4):721-727. doi: 10.1515/ap-2018-0085.
Taenia multiceps, one of the most widely distributed zoonotic tapeworm parasites, is able to parasitize the small intestine of canids. The metacestode of T.multiceps is fatal to ruminants and causes important economic losses in livestock. However, molecular characteristics of T.multiceps and coenurus in China are still unclear. In this study, 36 goat isolates of the coenurus stage and 18 dog isolates of the adult stage of T.multiceps were obtained from three geographical areas in China and the isolated parasite above were analyzed by amplifying the partial of cytochrome coxidase subunit 1(pcox1), 12S ribosomal RNA (12S rRNA) from mitochondrial DNA (mtDNA) regions and an internal transcribed spacer (ITS) of ribosomal DNA (rDNA). These DNA sequences obtained from T.multiceps and coenurus were employed to evaluate the nucleotide diversity and confirm the relationship between T.multiceps and coenurus. Sequences variation were 0-1.4%, 0-1.5%, 0-4.2% for pcox1, 12S rRNA and ITS, respectively, among T.multiceps and coenurus isolates obtained in this study. In Sichuan province, sequence variations for Coenurus cerebralis isolated from Yaan city were 0-1.4% for pcox1, 0-1.0% for 12S rRNA and 0-2.1% for ITS. In Hunan province, variations were 0-1.0%, 0-1.5% and 0-3.3% for corresponding genes for non-coenurus cerebralis isolated from Changsha city, while variations of T.multiceps isolates from Xiangxi autonomous prefecture were 0-1.0%, 0-1.1% and 0-3.4% for pcox1, 12S rRNA and ITS, respectively. Phylogenetic analysis based on pcox1 sequences indicated that all cerebral and noncerebral metacestodes belong to T.multiceps. These results provide reference values for future molecular epidemiological and biological study on T.multiceps in dogs and intermediate hosts.
多头绦虫是分布最广泛的人畜共患绦虫寄生虫之一，能够寄生于犬科动物的小肠。多头绦虫的中绦期对反刍动物是致命的，并给家畜造成重大经济损失。然而，中国多头绦虫和共尾蚴的分子特征仍不清楚。在本研究中，从中国三个地理区域获得了36株共尾蚴期的山羊分离株和18株多头绦虫成虫期的犬分离株，并通过扩增线粒体DNA（mtDNA）区域的细胞色素氧化酶亚基1（pcox1）、12S核糖体RNA（12S rRNA）的部分片段以及核糖体DNA（rDNA）的内部转录间隔区（ITS），对上述分离的寄生虫进行了分析。从多头绦虫和共尾蚴获得的这些DNA序列用于评估核苷酸多样性，并确定多头绦虫和共尾蚴之间的关系。在本研究获得的多头绦虫和共尾蚴分离株中，pcox1、12S rRNA和ITS的序列变异分别为0-1.4%、0-1.5%、0-4.2%。在四川省，从雅安市分离的脑多头蚴的pcox1序列变异为0-1.4%，12S rRNA为0-1.0%，ITS为0-2.1%。在湖南省，从长沙市分离的非脑多头蚴对应基因的变异分别为0-1.0%、0-1.5%和0-3.3%，而从湘西自治州分离的多头绦虫分离株的pcox1、12S rRNA和ITS变异分别为0-1.0%、0-1.1%和0-3.4%。基于pcox1序列的系统发育分析表明，所有脑型和非脑型中绦期均属于多头绦虫。这些结果为今后对犬和中间宿主中多头绦虫的分子流行病学和生物学研究提供了参考价值。
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