Department of Cariology, Operative Dentistry and Dental Public Health, Indiana University School of Dentistry, 415 Lansing St., Indianapolis, IN 46202, USA; Department of Restorative Dental Science, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.
Department of Cariology, Operative Dentistry and Dental Public Health, Indiana University School of Dentistry, 415 Lansing St., Indianapolis, IN 46202, USA.
Dent Mater. 2019 Oct;35(10):1464-1470. doi: 10.1016/j.dental.2019.07.018. Epub 2019 Aug 5.
Enamel thickness determination by Cross-Polarization Optical Coherence Tomography (CP-OCT) is a promising approach for quantitative monitoring of tooth wear progression. This study evaluated the ability of CP-OCT to quantify the thickness of natural enamel before, during and after tooth wear simulation.
Natural, unpolished human dental enamel slabs were submitted to five wear stages (Wear 1: to level the surfaces; Wear 2 to Wear 5: 0.05±0.02mm reduction each) simulated by an automatic grinding/polishing machine. Enamel thickness was evaluated with CP-OCT and a gold-standard method (micro-CT) at baseline and after every wear stage. Data were analyzed using ANOVA with pairwise comparisons for wear stages' impact on the thickness and wear depth measurements. The inter-method agreement was analyzed using intra-class correlation coefficients, the difference between means, and Bland-Altman plots.
Enamel thickness measurements (mean±standard error, in mm) with natural (1.40±0.05) and worn surfaces (1.08±0.02) by CP-OCT did not differ significantly from those measured by micro-CT (natural=1.39±0.05; worn=1.09±0.02; p-values=0.30 and 0.39, respectively). CP-OCT and micro-CT showed excellent agreement on natural (ICC=0.98) and worn surfaces (ICC=0.98) enamel thickness measurements. Among and between wear stages, there were significant differences in enamel thickness and wear depth measurements for both methods (p-value <0.0001 for all). Both methods yielded similar measurements' mean (0.14±0.01; p-value=0.87) and were in good agreement (ICC=0.77) for wear depth estimation.
CP-OCT allows accurate measurement of enamel thickness on natural tooth surfaces. Enamel thickness measurement by CP-OCT allows quantitative monitoring of enamel thickness changes and wear depth following progressive wear.
通过交叉偏振光相干断层扫描（CP-OCT）测定牙釉质厚度是定量监测牙齿磨损进展的一种很有前途的方法。本研究评估 CP-OCT 在模拟牙齿磨损前后定量测量天然牙釉质厚度的能力。
将未经抛光的天然人牙釉质薄片进行 5 个磨损阶段（磨损 1：使表面平整；磨损 2 至磨损 5：每次减少 0.05±0.02mm）的模拟，由自动研磨/抛光机完成。在基线和每个磨损阶段后，使用 CP-OCT 和金标准方法（微 CT）评估牙釉质厚度。采用方差分析和两两比较分析磨损阶段对厚度和磨损深度测量的影响。采用组内相关系数、均值差值和 Bland-Altman 图分析两种方法之间的一致性。
CP-OCT 测量的天然（1.40±0.05）和磨损表面（1.08±0.02）牙釉质厚度（平均值±标准误差，mm）与微 CT 测量值（天然=1.39±0.05；磨损=1.09±0.02；p 值分别为 0.30 和 0.39）无显著差异。CP-OCT 和微 CT 对天然（ICC=0.98）和磨损表面（ICC=0.98）牙釉质厚度测量均具有极好的一致性。两种方法在所有磨损阶段的牙釉质厚度和磨损深度测量均存在显著差异（所有 p 值均<0.0001）。两种方法在测量磨损深度方面具有相似的均值（0.14±0.01；p 值=0.87），且一致性良好（ICC=0.77）。
CP-OCT 可准确测量天然牙釉质表面的牙釉质厚度。CP-OCT 测量牙釉质厚度可定量监测牙釉质厚度变化和渐进性磨损后的磨损深度。
