Indiana University, School of Dentistry, Department of Cariology, Operative Dentistry and Dental Public Health, Indianapolis, IN, USA.
University of California, San Francisco School of Dentistry, Department of Preventive and Restorative Dental Sciences, San Francisco, CA, USA.
Braz Oral Res. 2023 Sep 4;37:e081. doi: 10.1590/1807-3107bor-2023.vol37.0081. eCollection 2023.
This study tested a novel in vitro dental erosion-abrasion model and the performance of cross-polarization optical coherence tomography (CP-OCT) in longitudinally monitoring the simulated lesions. Thirty human enamel specimens were prepared and randomized to receive three dental erosion-abrasion (EA) protocols: severe (s-EA, lemon juice/pH:2.5/4.25%w/v citric acid), moderate (m-EA, grapefruit juice/pH:3.5/1.03%w/v citric acid) and no-EA (water, control). EA challenge was performed by exposing the specimens to acidic solutions 4x/day and to brushing 2x/day with 1:3 fluoridated toothpaste slurry, for 14 days. Enamel thickness measurements were obtained using CP-OCT at baseline (D0), 7 (D7) and 14 days (D14) and micro-computed tomography (micro-CT) at D14. Enamel surface loss was measured with both CP-OCT and optical profilometry at D0, D7 and D14. Data was analyzed with repeated-measures ANOVA and Pearson's correlation (r) (α = 0.05). CP-OCT enamel thickness decreased over time in the s-EA group (D0 >D7 > D14, p < 0.001) and m-EA group (D0 > D14, p = 0.019) but did not change in the no-EA group (p = 0.30). Overall, CP-OCT and micro-CT results at D14 correlated moderately (r = 0.73). CP-OCT surface loss was highest for s-EA (p <0.001) but did not differ between moderate and no-EA (p = 0.25). Enamel surface loss with profilometry increased with severity (no-EA>m-EA>s-EA, p < 0.001). D14 surface loss was higher than D7 for both methods except for the no-EA group with profilometry. CP-OCT and profilometry had moderate overall correlation (r = 0.70). Our results revealed that the currently proposed in vitro dental erosion-abrasion model is valid and could simulate lesions of different severities over time. CP-OCT was a suitable method for monitoring the EA lesions.
本研究测试了一种新型的体外牙齿酸蚀磨损模型，以及交叉偏振光相干断层扫描（CP-OCT）在纵向监测模拟病变中的性能。将 30 个人类牙釉质标本制备并随机分为三组进行三种牙齿酸蚀磨损（EA）方案的实验：重度（s-EA，柠檬汁/pH：2.5/4.25%w/v 柠檬酸）、中度（m-EA，葡萄柚汁/pH：3.5/1.03%w/v 柠檬酸）和无酸蚀（水，对照）。通过每天 4 次暴露于酸性溶液和每天 2 次用 1:3 氟化牙粉浆刷牙来进行 EA 挑战，持续 14 天。在基线（D0）、7 天（D7）和 14 天（D14）时使用 CP-OCT 测量牙釉质厚度，并在 D14 时使用微计算机断层扫描（micro-CT）测量。在 D0、D7 和 D14 时使用 CP-OCT 和光学轮廓仪测量牙釉质表面损失。使用重复测量方差分析和 Pearson 相关（r）（α = 0.05）进行数据分析。CP-OCT 牙釉质厚度随着时间的推移在 s-EA 组（D0>D7>D14，p<0.001）和 m-EA 组（D0>D14，p=0.019）中减少，但在无酸蚀组中没有变化（p=0.30）。总体而言，D14 时 CP-OCT 和 micro-CT 的结果中度相关（r=0.73）。s-EA 的 CP-OCT 表面损失最高（p<0.001），但在中度和无酸蚀组之间没有差异（p=0.25）。与严重性相关，轮廓仪测量的牙釉质表面损失随着无酸蚀组>m-EA 组>s-EA 组（p<0.001）而增加。两种方法的 D14 表面损失均高于 D7，除了用轮廓仪测量的无酸蚀组。CP-OCT 和轮廓仪具有中度的总体相关性（r=0.70）。我们的结果表明，目前提出的体外牙齿酸蚀磨损模型是有效的，可以随着时间的推移模拟不同严重程度的病变。CP-OCT 是监测 EA 病变的一种合适方法。
