Chand P, Khanna R N, Sadana J R
Department of Veterinary Pathology, Haryana Agricultural University, Hissar, India.
J Appl Bacteriol. 1988 May;64(5):445-9. doi: 10.1111/j.1365-2672.1988.tb05101.x.
Counter-immunoelectrophoresis was employed for the detection of Brucella antigen in stomach contents of aborted buffalo fetuses and antibody in aborted as well as apparently healthy in contact buffaloes. Five of 16 aborted cases were serologically positive for brucellosis but isolation of Brucella abortus was successful in only two cases. By counter-immunoelectrophoresis, Brucella antigen was detected in the fetal stomach contents of four serologically positive cases. Of the 68 serum samples from in contact healthy buffaloes, 10 were positive with counter-immunoelectrophoresis: more than were detected by tube agglutination, Rose Bengal plate agglutination, complement fixation and agar gel precipitation test.
采用对流免疫电泳法检测流产水牛胎儿胃内容物中的布鲁氏菌抗原以及流产水牛和与其接触的看似健康的水牛体内的抗体。16例流产病例中有5例布鲁氏菌病血清学检测呈阳性，但仅2例成功分离出流产布鲁氏菌。通过对流免疫电泳法，在4例血清学阳性病例的胎儿胃内容物中检测到了布鲁氏菌抗原。在68份来自接触过的健康水牛的血清样本中，有10份对流免疫电泳检测呈阳性：比试管凝集试验、虎红平板凝集试验、补体结合试验和琼脂凝胶沉淀试验检测出的更多。
