MIIT Key Laboratory of Advanced Display Materials and Devices, School of Environmental and Biological Engineering , Nanjing University of Science and Technology , Nanjing 210094 , China.
Department of Biotechnology Engineering , Ben-Gurion University of the Negev , Beer-Sheva 84105 , Israel.
ACS Appl Mater Interfaces. 2019 Sep 4;11(35):32366-32372. doi: 10.1021/acsami.9b08566. Epub 2019 Aug 21.
Glycopolymers have emerged as powerful and versatile glycan analogues for the investigation of cellular signal transduction. In this study, a layer of the glycopolymer-brush (GlyB) interface was functionalized on the surface of gold substrates. In order to enhance the capability and accessibility of this transducer interface, a combined protocol of copper(0)-mediated living radical polymerization (Cu(0)-LRP) with subsequent "CuAAC" click reaction was utilized to synthesize a set of novel glycopolymer precursors with a tunable scaffold structure and pyranose ligands. The resulting glycopolymer exhibited a fine-tuned molecular weight with a minor dispersity of 1.27. Through surface plasmon resonance (SPR) analysis, various GlyB interfaces exhibiting different saccharide moieties (glucose, mannose, and galactose) were examined to study their adhesion or antiadhesion potential toward three types of proteins, concanavalin A, bovine serum albumin, and peanut agglutinin (PNA). The strong affinity between poly(galactose) and PNA was further employed to construct a proof-of-concept aggregation-mediated sensing system. This minimal naked-eye sensor that consisted of only two substances, namely, gold nanoparticles and glycopolymers, was characterized and tested for its potential in protein quantification.
糖聚合物已成为研究细胞信号转导的强大而多功能的聚糖类似物。在这项研究中，在金基底表面功能化了一层糖聚合物刷（GlyB）界面。为了增强这种传感器界面的能力和可及性，利用铜（0）介导的活性自由基聚合（Cu（0）-LRP）与随后的“CuAAC”点击反应的组合方案，合成了一系列具有可调支架结构和吡喃糖配体的新型糖聚合物前体。所得糖聚合物表现出精细调节的分子量和 1.27 的较小分散性。通过表面等离子体共振（SPR）分析，研究了具有不同糖基部分（葡萄糖、甘露糖和半乳糖）的各种 GlyB 界面，以研究它们对三种蛋白质（刀豆球蛋白 A、牛血清白蛋白和花生凝集素（PNA）的粘附或抗粘附潜力。聚（半乳糖）与 PNA 之间的强亲和力进一步被用于构建聚集介导的传感系统的概念验证。这个最小的肉眼传感器仅由两种物质，即金纳米粒子和糖聚合物组成，对其进行了表征，并测试了其在蛋白质定量方面的潜力。
