Walker A P, Bachelard H S
Division of Biochemistry, U.M.D.S., St. Thomas's Hospital, London, England.
J Neurochem. 1988 Nov;51(5):1394-9. doi: 10.1111/j.1471-4159.1988.tb01103.x.
Methods for studying breaks in DNA strands and their repair, originally developed for prokaryotes and cultured cell lines, have been applied to preparations from rat brain. The relative sensitivities of these methods, which include alkaline sucrose density gradient sedimentation, nucleoid sedimentation, and ADP-ribosyltransferase assay, are compared.
最初为原核生物和培养细胞系开发的研究DNA链断裂及其修复的方法,已应用于大鼠脑组织提取物。对这些方法的相对敏感性进行了比较,这些方法包括碱性蔗糖密度梯度沉降、核沉降和ADP-核糖基转移酶测定。