Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.
Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.
Int J Biol Macromol. 2019 Nov 1;140:477-483. doi: 10.1016/j.ijbiomac.2019.08.041. Epub 2019 Aug 6.
Aquaculture systems are known to be major reservoirs of Aeromonas hydrophila. The use of Chinese medicinal polysaccharides has become a successful strategy to prevent bacterial diseases in aquaculture. A water-soluble polysaccharide, HP-02, was purified from honeysuckle flowers, and the present study was aimed to examine its immunomodulatory and anti-A. hydrophila effects in Cyprinus carpio L. The results showed that the molecular weight of HP-02 was estimated to be 38 kDa. Monosaccharide composition was determined to be arabinose, rhamnose, mannose, glucose, and galactose in a molar ratio of 2.5: 1.8: 3.6: 3.7: 1.9. HP-02 not only distinctly improved the proliferation and phagocytosis of head kidney cells, but also exerted significant immunoregulation activity by increasing the secretion of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 and the anti-inflammatory cytokines IL-10 and TGF-β at the protein level in head kidney cells and serum. Moreover, during pathogen infection in vivo, HP-02 significantly reduced the expression of pro-inflammatory cytokines and increased the expression of anti-inflammatory cytokines. These results indicated that HP-02 had immunomodulatory effects on common carp both in vitro and in vivo, and HP-02 can be applied as a common carp feed supplement to enhance fish immunity and disease resistance against A. hydrophila.
水产养殖系统是水生嗜水气单胞菌的主要储存库。使用中药多糖已成为预防水产养殖中细菌疾病的成功策略。从金银花中纯化出一种水溶性多糖 HP-02，本研究旨在研究其对鲤鱼的免疫调节和抗嗜水气单胞菌作用。结果表明，HP-02 的分子量估计为 38 kDa。单糖组成分析表明，HP-02 由阿拉伯糖、鼠李糖、甘露糖、葡萄糖和半乳糖组成，摩尔比为 2.5:1.8:3.6:3.7:1.9。HP-02 不仅明显提高了头肾细胞的增殖和吞噬作用，而且通过增加头肾细胞和血清中促炎细胞因子 TNF-α、IL-1β、IL-6 和 IL-12 以及抗炎细胞因子 IL-10 和 TGF-β 的分泌，发挥显著的免疫调节活性。此外，在体内病原体感染期间，HP-02 显著降低了促炎细胞因子的表达，增加了抗炎细胞因子的表达。这些结果表明，HP-02 对鲤鱼具有体内外的免疫调节作用，HP-02 可作为鲤鱼饲料添加剂，增强鱼类对嗜水气单胞菌的免疫力和抗病力。
