Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; Jiangsu Provisional Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
Bioresour Technol. 2019 Nov;292:121897. doi: 10.1016/j.biortech.2019.121897. Epub 2019 Jul 26.
Co-production of α-ketoglutaric acid (KGA) and pyruvic acid (PYR) by Yarrowia lipolytica WSH-Z06 could significantly increase the final titer and yield of keto acids. However, efficient separation of KGA and PYR in an economic manner is a big challenge owing to their similar properties. In the present study, a separation process was established to convert PYR in the fermentation broth to l-tyrosine (TYR). Owing to its low solubility, TYR was easily precipitated out and could be easily removed from the reaction system. The whole-cell catalysis reaction solution was subjected to acid treatment, centrifugation, cation exchange column separation, rotary evaporation, Buchner funnel filtration, and dry separation method to obtain KGA and TYR powders. The purity/recovery rates of KGA and TYR were 98.16%/78.68% and 98.19%/73.46%, respectively. The use of biological pathways to separate KGA from the culture broth could make the separation process easier and further decrease the operation cost.
解脂耶氏酵母 WSH-Z06 共生产 α-酮戊二酸（KGA）和丙酮酸（PYR）可以显著提高酮酸的最终浓度和产率。然而，由于它们的性质相似，因此以经济的方式有效地分离 KGA 和 PYR 是一个巨大的挑战。在本研究中，建立了一种分离工艺，将发酵液中的 PYR 转化为 L-酪氨酸（TYR）。由于 TYR 的溶解度低，它很容易沉淀出来，并可以很容易地从反应体系中去除。全细胞催化反应溶液经过酸处理、离心、阳离子交换柱分离、旋转蒸发、布氏漏斗过滤和干燥分离方法，得到 KGA 和 TYR 粉末。KGA 和 TYR 的纯度/收率分别为 98.16%/78.68%和 98.19%/73.46%。使用生物途径从培养液中分离 KGA 可以使分离过程更容易，并进一步降低操作成本。
