Lu Qiyuan, Shan Xiaoyu, Zeng Weizhu, Zhou Jingwen
Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
Bioresour Bioprocess. 2022 Oct 17;9(1):109. doi: 10.1186/s40643-022-00593-5.
Pyruvic acid is an important organic acid and a key industrial raw material. It is widely used in the chemical, agricultural, and food fields. Candida glabrata is the preferred strain for pyruvic acid production. The waste yeast cell for pyruvic acid fermentation with C. glabrata are rich in protein, amino acid, nucleic acid, and vitamins, as potential and cost-effective nitrogen source raw material. In this study, the potential of C. glabrata to produce pyruvic acid using spent yeast cell dry powder was evaluated. When 30 g/L of spray-dried spent yeast cell powder was used as the seed nitrogen source, a high titer of pyruvic acid was obtained. The pyruvic acid production reached 63.4 g/L with a yield of 0.59 g/g in a 5 L bioreactor. After scale-up to a 50 L bioreactor using the fermented spent yeast cell dry powder as a seed nitrogen source, 65.1 g/L of pyruvic acid was harvested, with a yield of 0.61 g/g. This study proposes a promisingapproach for increasing the pyruvic acid titer and reducing the costs.
丙酮酸是一种重要的有机酸和关键的工业原料。它广泛应用于化工、农业和食品领域。光滑念珠菌是生产丙酮酸的首选菌株。用于光滑念珠菌丙酮酸发酵的废弃酵母细胞富含蛋白质、氨基酸、核酸和维生素，是潜在的、具有成本效益的氮源原料。在本研究中，评估了光滑念珠菌利用废酵母细胞干粉生产丙酮酸的潜力。当使用30 g/L的喷雾干燥废酵母细胞粉作为种子氮源时，获得了高滴度的丙酮酸。在5 L生物反应器中，丙酮酸产量达到63.4 g/L，产率为0.59 g/g。在扩大到50 L生物反应器并使用发酵后的废酵母细胞干粉作为种子氮源后，收获了65.1 g/L的丙酮酸，产率为0.61 g/g。本研究提出了一种提高丙酮酸滴度和降低成本的有前景的方法。
