Shizuoka University, Graduate School of Science and Technology, Hamamatsu, 4328561, Japan; Shizuoka University, Research Institute of Electronics, Hamamatsu, 4328011, Japan.
Shizuoka University, Graduate School of Science and Technology, Hamamatsu, 4328561, Japan.
Ultramicroscopy. 2019 Nov;206:112823. doi: 10.1016/j.ultramic.2019.112823. Epub 2019 Aug 1.
Cell stimulation has been performed with a focused electron beam. To protect the live cells from the vacuum environment of the electron beam, the beam irradiated the ambient cells via a thin film. In this way, the cells were electrically stimulated with nanometre resolution in a non-contact process. The response of calcium ion concentration in a single HeLa cell after electron beam irradiation was examined. This technique has the potential to stimulate single ion channels, granules, and organelles.
细胞刺激是通过聚焦电子束来实现的。为了保护活细胞免受电子束的真空环境影响，电子束通过薄膜照射周围的细胞。通过这种方式，细胞在非接触过程中以纳米级分辨率进行电刺激。研究了电子束辐照后单个 HeLa 细胞中钙离子浓度的响应。该技术具有刺激单个离子通道、颗粒和细胞器的潜力。
