Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Technology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530008, China.
Mar Drugs. 2019 Aug 8;17(8):463. doi: 10.3390/md17080463.
Angiotensin-I-converting enzyme (ACE) inhibitory peptides derived from natural products have shown a blood pressure lowering effect with no side effects. In this study, two novel ACE inhibitory peptides (His-Leu-His-Thr, HLHT and Gly-Trp-Ala, GWA) were purified from pearl oyster () meat protein hydrolysate with alkaline protease by ultrafiltration, polyethylene glycol methyl ether modified immobilized metal ion affinity medium, and reverse-phase high performance liquid chromatography. Both peptides exhibited high ACE inhibitory activity with IC values of 458.06 ± 3.24 μM and 109.25 ± 1.45 μM, respectively. Based on the results of a Lineweaver-Burk plot, HLHT and GWA were found to be non-competitive inhibitor and competitive inhibitor respectively, which were confirmed by molecular docking. Furthermore, the pearl oyster meat protein hydrolysate exhibited an effective antihypertensive effect on SD rats. These results conclude that pearl oyster meat protein is a potential resource of ACE inhibitory peptides and the purified peptides, HLHT and GWA, can be exploited as functional food ingredients against hypertension.
从天然产物中衍生出的血管紧张素转化酶（ACE）抑制肽具有降血压作用且无副作用。在本研究中，两种新型 ACE 抑制肽（His-Leu-His-Thr，HLHT 和 Gly-Trp-Ala，GWA）通过超滤、聚乙二醇甲基醚修饰的固定化金属离子亲和介质和反相高效液相色谱法从珍珠蚝肉蛋白水解物中的碱性蛋白酶中得到纯化。这两种肽均表现出较高的 ACE 抑制活性，IC 值分别为 458.06 ± 3.24 μM 和 109.25 ± 1.45 μM。根据 Lineweaver-Burk 图的结果，HLHT 和 GWA 分别被发现为非竞争性抑制剂和竞争性抑制剂，这通过分子对接得到了证实。此外，珍珠蚝肉蛋白水解物对 SD 大鼠具有有效的降压作用。这些结果表明，珍珠蚝肉是 ACE 抑制肽的潜在资源，纯化得到的肽 HLHT 和 GWA 可作为防治高血压的功能性食品成分。
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