酶在两亲性豆蔻酰苯丙氨酸水凝胶中的包埋。

Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, M5S 3E5, Toronto, Canada.

Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1065 Military Trail, M1C 1A4, Scarborough, Canada.

Molecules. 2019 Aug 8;24(16):2884. doi: 10.3390/molecules24162884.

Supramolecular amino acid and peptide hydrogels are functional materials with a wide range of applications, however, their ability to serve as matrices for enzyme entrapment have been rarely explored. Two amino acid conjugates were synthesized and explored for hydrogel formation. These hydrogels were characterized in terms of strength and morphology, and their ability to entrap enzymes while keeping them active and reusable was explored. It was found that the hydrogels were able to successfully entrap two common and significant enzymes-horseradish peroxidase and -amylase-thus keeping them active and stable, along with inducing recycling capabilities, which has potential to further advance the industrial biotransformation field.

超分子氨基酸和肽水凝胶是具有广泛应用的功能材料，然而，它们作为酶固定基质的能力很少被探索。合成了两种氨基酸缀合物并探索了其水凝胶的形成。从强度和形态方面对这些水凝胶进行了表征，并探索了它们在保持酶活性和可重复使用的同时包埋酶的能力。结果发现，水凝胶能够成功地包埋两种常见且重要的酶——辣根过氧化物酶和α-淀粉酶——从而保持它们的活性和稳定性，同时诱导循环能力，这有可能进一步推动工业生物转化领域的发展。