Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
J Cell Physiol. 2020 Feb;235(2):1556-1567. doi: 10.1002/jcp.29074. Epub 2019 Aug 9.
The extracellular matrix of different mammalian tissues is commonly used as scaffolds in the field of tissue engineering. One of these tissues, which has frequently been studied due to its structural and biological features, is the small intestine submucosal membrane. These research are mainly done on the porcine small intestine. However, a report has recently been published about a scaffold produced from the submucosal layer of the ovine small intestine. In the present study, ovine small intestine submucosal (OSIS) was decellularized in a modified manner and its histological, morphological, and biomechanical properties were studied. Decellularization was performed in two phases: physical and chemical. In this method, a chloroform-methanol mixture, enzymatic digestion, and a constant dose of sodium dodecyl sulfate (SDS) was used in the least agitation time and its histological property and biocompatibility were evaluated in the presence of adipose tissue-derived stem cells (ADSCs); furthermore, ADSCs were isolated with a simple method (modified physical washing non-enzymatic isolation). The results were showed that the use of OSIS could be effective and operative. Mechanical properties, histological structure and shape, and glycosaminoglycan content were preserved. In the SDS-treated group, more than 90% of the native cells of tissue were deleted, and also in this group, no toxicity was observed and cell proliferation was supported, compared to the untreated group. Therefore, our results indicate that ADSCs seeded on OSIS scaffold could be used as a new approach in regenerative medicine as hybrid or hydrogel application.
不同哺乳动物组织的细胞外基质通常被用作组织工程领域的支架。其中一种组织由于其结构和生物学特性而经常被研究，即小肠黏膜下层。这些研究主要是在猪的小肠上进行的。然而，最近有一篇报道介绍了一种源自绵羊小肠黏膜下层的支架。在本研究中，我们以改良的方式对绵羊小肠黏膜下层（OSIS）进行脱细胞处理，并研究了其组织学、形态学和生物力学特性。脱细胞化分两个阶段进行：物理和化学。在这种方法中，使用氯仿-甲醇混合物、酶消化和恒定剂量的十二烷基硫酸钠（SDS），并在最少搅拌时间下进行，同时在脂肪组织来源的干细胞（ADSCs）存在下评估其组织学特性和生物相容性；此外，还使用一种简单的方法（改良的物理洗涤非酶分离）分离 ADSCs。结果表明，使用 OSIS 是有效和可行的。力学性能、组织学结构和形状以及糖胺聚糖含量得以保留。在 SDS 处理组中，超过 90%的组织天然细胞被删除，与未处理组相比，在该组中观察到没有毒性并且支持细胞增殖。因此，我们的结果表明，在 OSIS 支架上接种 ADSCs 可作为再生医学中的一种新方法，用于混合或水凝胶应用。
