Razaka H, Villani G, Hoffman J S, Defais M, Johnson N P
Laboratoire de Pharmacologie et de Toxicologie Fondamentales du Centre Nationale de la Recherche Scientifique, Toulouse, France.
Mutat Res. 1988 Sep-Oct;209(1-2):63-6. doi: 10.1016/0165-7992(88)90112-1.
Wild-type Escherichia coli were treated with cis-dichlorodiammineplatinum(II) (DDP) and then kept in a non-dividing state for 4 h. This post-treatment incubation did not increase survival, excision of platinum-DNA lesions was not observed, and post-treatment exposure to thiourea had no effect on the toxicity of DDP. In contrast, the mutation frequency increased by a factor of 3 and reached a plateau after 3 h incubation at 37 degrees C. The quantity of DDP on the bacterial DNA remained constant during this time. Post-treatment exposure to thiourea (which is reported to react with monofunctional platinum-DNA adducts) inhibited but did not reverse the increased mutagenicity. These results may reflect the evolution of monofunctional platinum-DNA lesions into bifunctional mutagenic adducts during post-treatment incubation of bacteria which have been exposed to DDP.
野生型大肠杆菌用顺二氯二氨铂（II）（DDP）处理，然后在非分裂状态下保持4小时。这种处理后的孵育并未提高存活率，未观察到铂-DNA损伤的切除，并且处理后暴露于硫脲对DDP的毒性没有影响。相比之下，突变频率增加了3倍，并在37℃孵育3小时后达到平台期。在此期间，细菌DNA上的DDP量保持恒定。处理后暴露于硫脲（据报道可与单功能铂-DNA加合物反应）可抑制但不能逆转增加的致突变性。这些结果可能反映了在暴露于DDP的细菌处理后孵育期间，单功能铂-DNA损伤演变为双功能诱变加合物的过程。
