Germanier M, Defais M, Johnson N P, Villani G
Mutat Res. 1985 Jan-Mar;145(1-2):35-41. doi: 10.1016/0167-8817(85)90037-9.
The adaptive response is an inducible DNA-repair system which diminishes the mutagenic and toxic effects of alkylating agents. A mutant of E. coli constitutive for adaptative repair, BS21, has been isolated. A spontaneous revertant of this strain, BS23, lacks the adaptive response. When compared to its wild-type parent, mutant BS21 showed an increased resistance to the killing and mutagenic effects of a compound which is not a classical alkylating agent, the antitumor drug cis-diamminedichloroplatinum(II) (cis-DDP). However, this resistance to cis-DDP was also found in strain BS23 which lacks the adaptive response. cis-DDP bound to the DNA of all 3 strains with the same efficiency. In addition, we have investigated the effect of UV radiation and we failed to observe a significant difference in the survival and mutagenesis of these strains. This evidence suggests that the resistance of BS21 and BS23 strains to cis-DDP is not a consequence of the adaptive response or increased excision repair.
适应性反应是一种可诱导的DNA修复系统，它能减少烷化剂的诱变和毒性作用。已分离出一株对适应性修复呈组成型的大肠杆菌突变体BS21。该菌株的一个自发回复突变体BS23缺乏适应性反应。与野生型亲本相比，突变体BS21对一种并非经典烷化剂的化合物——抗肿瘤药物顺二氨二氯铂（II）（顺铂，cis-DDP）的杀伤和诱变作用表现出更高的抗性。然而，在缺乏适应性反应的菌株BS23中也发现了这种对顺铂的抗性。顺铂以相同效率与所有3个菌株的DNA结合。此外，我们研究了紫外线辐射的影响，未观察到这些菌株在存活率和诱变方面有显著差异。这一证据表明，BS21和BS23菌株对顺铂的抗性并非适应性反应或增强的切除修复的结果。
