Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin si, Gyeonggi do 17104, Republic of Korea.
Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin si, Gyeonggi do 17104, Republic of Korea; Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin si, Gyeonggi do 17104, Republic of Korea.
Fitoterapia. 2019 Oct;138:104295. doi: 10.1016/j.fitote.2019.104295. Epub 2019 Aug 7.
Gynostemma pentaphyllum is a traditional oriental medicinal herb used as tea since ancient time. Among Gynostemma species, G. pentaphyllum has more active chemical components and better therapeutic effect. It is used to cure depression, diabetes, anxiety, hyperlipidemia, fatigue, immunity, cancer, and oxidative stress. Overexploitation of G. pentaphyllum for its medicinal benefits has been on a rise, due to which they are adulterated or mistakenly identified with other members of Gynostemma species. Hence, we used chloroplast universal regions such as ycf3, accD, petD, psbB and their polymorphism to distinguish G. pentaphyllum from other Gynostemma species. By using the species-specific primers derived from the above regions, we established a multiplex allele-specific PCR for the authentication of G. pentaphyllum from other species. Thus the PCR reaction produced unique amplicons of size 244 bp and 438 bp for G. pentaphyllum amplified by the primers flanking ycf3, and accD regions respectively. While a 607 bp, and 787 bp amplicons from the primers targeting psbB, and petD regions distinguished G. longipes, G. burmanicum, and G. pubescens species. Moreover, these primers were successful to analyze the dried tea samples of Gynostemma as well. Thus, the developed molecular markers could authenticate different Gynostemma species as well as its products thereby preventing the mistaken-identity of this medicinal herb.
绞股蓝是一种传统的东方药用草本植物，自古以来就被用作茶。在绞股蓝属中，绞股蓝含有更多的活性化学成分，具有更好的治疗效果。它被用于治疗抑郁症、糖尿病、焦虑症、高血脂、疲劳、免疫力、癌症和氧化应激。由于绞股蓝具有药用价值，对其的过度开发导致它被掺假或与绞股蓝属的其他成员混淆。因此，我们使用叶绿体通用区域，如 ycf3、accD、petD、psbB 及其多态性来区分绞股蓝和其他绞股蓝属物种。通过使用上述区域衍生的物种特异性引物，我们建立了一种多重等位基因特异性 PCR，用于鉴定绞股蓝与其他物种的区别。因此，PCR 反应产生了大小为 244 bp 和 438 bp 的独特扩增子，分别由侧翼 ycf3 和 accD 区域的引物扩增。而针对 psbB 和 petD 区域的引物则产生了大小为 607 bp 和 787 bp 的扩增子，可区分长梗绞股蓝、光叶绞股蓝和绞股蓝。此外，这些引物还成功地分析了绞股蓝的干茶样品。因此，开发的分子标记物可以鉴定不同的绞股蓝属物种及其产品，从而防止这种药用植物被错误识别。
