State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China.
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China; Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Virology. 2019 Oct;536:49-57. doi: 10.1016/j.virol.2019.07.027. Epub 2019 Jul 30.
Molecular adjuvants are vaccine delivery vehicle to increase specific antigens effectiveness. Herein, we concentrated on IgG Fc, an effective molecular adjuvant, to develop novel pseudorabies virus (PRV) subunit vaccines. Two major protective antigen genes of PRV were constructed and linked into the mouse IgG Fc fragment. The gD, gD-IgG2aFc, gB and gB-IgG2aFc proteins were expressed using a baculovirus system. Mice intranasally immunized with gD-IgG2aFc or gB-IgG2aFc subunit vaccine exhibited significantly higher PRV-specific antibodies, neutralizing antibodies and intracellular cytokines than the mice intranasally immunized with gD or gB subunit vaccine. Moreover, no histopathological lesions were observed in mice immunized with gB-IgG2aFc subunit vaccine via histopathology examination. Further, the gB-IgG2aFc subunit vaccine was efficient for PRV infection compared with live attenuated vaccine. Overall, these results suggest that IgG2a Fc fragment, as a potential molecular adjuvant, fused with PRV antigen might be a promising and efficient PRV vaccine candidate.
分子佐剂是一种疫苗输送载体，可提高特定抗原的有效性。在这里，我们专注于 IgG Fc，一种有效的分子佐剂，以开发新型伪狂犬病病毒 (PRV) 亚单位疫苗。构建了 PRV 的两个主要保护性抗原基因，并将其连接到小鼠 IgG Fc 片段上。使用杆状病毒系统表达 gD、gD-IgG2aFc、gB 和 gB-IgG2aFc 蛋白。通过鼻内免疫，用 gD-IgG2aFc 或 gB-IgG2aFc 亚单位疫苗免疫的小鼠比用 gD 或 gB 亚单位疫苗免疫的小鼠产生了更高水平的 PRV 特异性抗体、中和抗体和细胞内细胞因子。此外，通过组织病理学检查，未观察到用 gB-IgG2aFc 亚单位疫苗免疫的小鼠有组织病理学病变。此外，与活减毒疫苗相比，gB-IgG2aFc 亚单位疫苗对 PRV 感染更有效。总体而言，这些结果表明，IgG2a Fc 片段作为一种潜在的分子佐剂，与 PRV 抗原融合可能是一种有前途和有效的 PRV 疫苗候选物。
